Correlation between expression of β2 adrenergic receptors and HER2, K-ras genes in breast cancer / 中华内分泌外科杂志

ABSTRACT

Objective To investigate the expression of β2 adrenergic receptor (β2 AR) in invasive ductal carcinoma of the breast,and their correlation with HER2 and K-ras genes in breast cancer.Methods The expression of β2 AR,HER2 and K-ras gene in 10 cases of normal breast tissues and 33 cases of invasive ductal carcinoma of the breast were detected by immunohistochemical staining of streptomycin antibiotin protein and peroxidase assay (SP).Such statistical methods as SPSS17.0 statistical software,Mann-Whitney test and Spearman rank correlation test were applied to process and analyze the experimental data.Results β2 AR was expressed in all breast cancer cells,with an overexpression rate of 21/33.The positive expression rate of HER2 in breast cancer cells was 23/33,with an overexpression rate of 20/33.K-ras was positively expressed in breast cancer tissue(28/33),with an overexpression rate of 11/33.All of these three were significantly higher than those in the normal breast tissues.Z value of β-2 AR expression was 4.876,P value was 0.000.Z value of HER2 expression was 2.752,and P value was 0.006.Z value was 3126,and P value was 0.020 for K-ras expression.In the study of the correlation of β2 AR expression with HER2 and K-ras,the expression of β2 AR and HER2 and the expression of β2 AR and K-ras were correlated.The difference in expressions of β2 AR was statistically significant only in groups with or without lymph node metastasis,but not in groups varied in age,tumor size,clinical stage,pathological grading or hormone level.Conclusion The high expression of β2 AR,HER2,and K-ras in breast cancer tissues is associated with the occurrence and development of breast cancer.There is also a greater risk of potential lymph node metastasis in breast cancer with high expression of β-2 AR.β-2 AR may play a role in pathogenesis of breast cancer through HER2 and K-ras.