Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles / 대한수의학회지
Korean Journal of Veterinary Research
; : 81-88, 2019.
Article
in Ko
| WPRIM
| ID: wpr-760351
Responsible library:
WPRO
ABSTRACT
This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for two days in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovine serum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the mean diameters of the SAF treated with FBS, PVA, and no IVG-MAF (113.0–114.8 µm) were significantly larger than that of no IVG-SAF (111.8 µm). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar to that in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyte glutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%), PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFF and FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Oocytes
/
Parthenogenesis
/
Polyvinyl Alcohol
/
Blastocyst
/
In Vitro Techniques
/
Serum Albumin, Bovine
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Follicular Fluid
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Embryonic Development
/
Glutathione
/
Metaphase
Limits:
Pregnancy
Language:
Ko
Journal:
Korean Journal of Veterinary Research
Year:
2019
Document type:
Article
Publication country:
COREA DEL SUR
/
COREIA DO SUL
/
KR
/
SOUTH KOREA