Determination of methylenedianiline in urine by high performance liquid chromatography-tandemmass spectrometry / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 308-311, 2018.
Article
in Zh
| WPRIM
| ID: wpr-806314
Responsible library:
WPRO
ABSTRACT
Objective@#To develop a method for determination of metabolites of diphenylmethane diisocyanate (MDI) in urine, i.e. methylenedianiline (MDA) by high performance liquid chromatography-tandem mass (LC-MS-MS) . @*Methods@#Urine samples were prepared by hydrolyzation with sulfuric acid and extraction by acetonitrile, and then separated on a Shim-pack XR-ODS column, analyzed with high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) . The external solvent standard calibration were tested. @*Results@#The linearity ranges were 0.05~20.00 μg/L, The related coefficients were 0.999 5. The limit of detection was 0.02 μg/L. The rats of recovery were 91.0%~103.4%. The relative standard deviations were between 2.7%~7.3%. @*Conclusion@#The method was sensitive, accurate and suitable for the MDA determination in urine of MDI exposed population.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2018
Document type:
Article