Effect of silencing the VDR gene on the migration and invasion of prostate cancer cells / 中华男科学杂志
National Journal of Andrology
; (12): 969-974, 2017.
Article
in Zh
| WPRIM
| ID: wpr-812848
Responsible library:
WPRO
ABSTRACT
Objective@#To investigate the effect of small interfering RNA silencing the vitamin D receptor (VDR) on the biological behavior of prostate cancer PC-3 cells.@*METHODS@#We constructed the VDR-shRNA lentiviral vector and determined the mRNA and protein expressions of VDR by RT-PCR and Western blot. Using scratch wound healing and Transwell chamber assays, we detected the changes in the migration and invasiveness of the PC-3 cells after silencing VDR.@*RESULTS@#The VDR-shRNA plasmid significantly interfered the VDR expression and successfully screened the cell lines with stable VDR-shRNA interference. The rate of scratch wound healing was markedly lower in the VDR interference group than in the blank control and LV3 negative control groups (59% vs 73.6% and 77.8%, P 0.05), and so was the count of permeable cells (P 0.05). The migration ability and invasiveness of the VDR-treated cells were remarkably decreased as compared with those of the control cells.@*CONCLUSIONS@#Down-regulated expression of the VDR gene may reduce the migration and invasiveness of prostate cancer cells.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Plasmids
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Prostatic Neoplasms
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Wound Healing
/
RNA, Messenger
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Transfection
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Down-Regulation
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Cell Movement
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Receptors, Calcitriol
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Lentivirus
Limits:
Humans
/
Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2017
Document type:
Article