Dynamic changes of cellular HIV-1 DNA quantification during highly active antiretroviral therapy in Chinese HIV infected individuals / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 537-544, 2013.
Article
in Zh
| WPRIM
| ID: wpr-814846
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the dynamic changes of 3 types of viral reservoir cells (NK cells, T lymphocytes and monocytes), and its relationship with treatment effect in Chinese HIV-1 infected patients receiving highly active antiretroviral treatment (HAART) for 2 years.@*METHODS@#A total of 40 chronic HIV-1-infected adults who initiated HAART were enrolled in this study and followed up for 2 years. Peripheral whole blood was obtained from each patient at baseline (0 month), 6, 12, 18 and 24 months. Real-time fluorescent quantitative PCR was used to detect the HIV-1 RNA in the plasma and HIV-1 DNA in NK cells, T lymphocytes and monocytes. All the data were statistically analyzed.@*RESULTS@#CD4 count increased with the decrease of the viral load during HAART. After HAART initiation, HIV-1 DNA showed a significant decrease in NK cells, T lymphocytes and monocytes. The HIV-1 DNA from T lymphocytes, NK cells and monocytes correlated positively with the HIV- 1 RNA (P<0.05) while NK cells and T lymphocytes correlated negatively with CD4+ T cell count. However we did not find significant correlation between CD4+ T cell count and HIV-1 DNA in monocytes at the baseline of HAART.@*CONCLUSION@#This study found that NK cell was an important HIV cellular reservoir besides T lymphocytes and monocytes. T lymphocytes may be the main long lasting HIV reservoir. HIV-1 proviral DNA may play an important role in the efficacy of treatment and monitoring the disease progression.
Full text:
1
Database:
WPRIM
Main subject:
Virology
/
Blood
/
DNA, Viral
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RNA, Viral
/
Killer Cells, Natural
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Monocytes
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T-Lymphocytes
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HIV Infections
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HIV-1
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Viral Load
Limits:
Adult
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Female
/
Humans
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Male
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2013
Document type:
Article