Expression of recombined human immunoglobulin λ light chain 022 and TOM1 mRNA in omental adipose tissues of patients with type 2 diabetes and insulin resistance / 第二军医大学学报

ABSTRACT

Objective: To investigate the expression of recombined human immunoglobulin λ light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance (T2DM-IR). Methods: Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained. The mRNA levels of recombined human immunoglobulin λ light chain 022 and TOM1 were measured by fluorescent quantitative RT-PCR; expression of HSIGVL022 protein was identified by immunohistochemistry. Results: The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were (34 140±6 160) copy/million house-keeping genes and (4 440±617) copy/ million housekeeping genes, respectively; those in control group were (5 930±661) copy/million house-keeping genes and (1 360±82) copy/million house-keeping genes, respectively. There were significant difference between the 2 groups (P<0.05). The mRNA level of HSIGVL022 was significantly higher than that of TOM1 (P<0.01) and was linearly correlated with homeostatic model assessment-insulin resistance (HOMA-IR) index(P<0.05). The positive rates of HSIGVL022 protein in T2DM-IR and control group were (12.43±2.41)% and (2.31±0.48)%, respectively; the strong positive rates were (6.62±1.69)% and (2.12± 0.34)%, respectively (P<0.05). Conclusion: Both HSIGVL022 and TOM1 are expressed in the fat tissues of greater omentum, with the level of HSIGVL022 higher than that of TOM1. The 2 genes are up-regulated in patients with insulin resistance and the HSIGVL022 protein increases correspondingly. The mRNA level of HSIGVL022 is linearly correlated with HOMA-IR index. The 2 genes are possibly related with insulin resistance in type 2 diabetes.