AZD5363 inhibits cell proliferation, migration and induces apoptosis of breast cancer MDA-MB-231 cells / 肿瘤

ABSTRACT

Objective: To investigate the effects of AZD5363, an inhibitor of protein kinase B (Akt), on proliferation, migration and apoptosis of human breast cancer cell line MDA-MB-231, and to further clarify their possible molecular mechanisms Methods: After treatment with different concentrations (0.5, 1, 5, 10, 20 and 50 μmol/L) of AZD5363, the viability of MDA-MB-231 cells was detected by MTT assay, the cell cycle distribution was analyzed by FCM, the cell migration ability was detected by wound healing test and Transwell chamber assay, the cell apoptosis rate was detected by TUNEL method. Then the expression levels of cell cycle- and apoptosis-related proteins were measured by Western blotting. Results: AZD5363 suppressed the cell viability in a dose-dependent manner (P < 0.05), and arrested the cell cycle progression at S phase by up-regulating the expression of p53 and down-regulating the expression of cyclin B1 (all P < 0.05). AZD5363 significantly inhibited the cell migration (P < 0.05), and induced the cell apoptosis (P < 0.05) by activating caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins (both P < 0.05). Conclusion: AZD5363 can inhibit cell activity and migration, and induce apoptosis of human breast cancer cell line MDA-MB-2 31, thereby exhibiting its anticancer activity.