Catalytic conversion of protopanaxadiol saponins to 20(R)-ginsenoside Rg3 by tartaric acid / 中草药

ABSTRACT

Objective: To prepare 20(R)-ginsenoside Rg3 selectively and to provide the theory basis for the preparation. Methods: 20(R)-Ginsenoside Rg3 was prepared by hydrolyzing protopanaxadiol (PPD)-type saponins using tartaric acid as the catalyst. The preparation condition was optimized by one-factor experiment and orthogonal test, and the reaction products were analyzed by HPLC. Results: The optimization result of orthogonal test showed that when PPD-type saponins (10 mg/mL) were hydrolyzed by tartaric acid (1.5 mol/L) at 110°C for 2.5 h, all the ginsenosides Rb1, Rc, Rb2, Rb3, and Rd were converted, the yields of 20(R)-ginsenoside Rg3 was 50.15%, and the diastereomer excess percentage (de%) was 93.12%. Conclusion: This method is simple, low-cost, and suitable for the mass production, which is very important to promote the study on the pharmacological activities of 20(R)-ginsenoside Rg3.