Research on in vivo metabolites of Longxue Tongluo Capsules by liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectrometry / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4841-4848, 2021.
Article
in Zh
| WPRIM
| ID: wpr-888192
Responsible library:
WPRO
ABSTRACT
Longxue Tongluo Capsules(LTC) has good efficacy against blood stasis syndrome during the recovery period of ischemic stroke. Its main active ingredient is the phenolic extract of Chinese dragon's blood. In our previous study, the primary mass fragmentation pathways of phenolic derivatives from LTC were clarified. Herein, the metabolites in rat plasma were characterized following the oral administration of loureirin A and loureirin C using liquid chromatography coupled with hybrid ion trap/time-of-flight mass spectro-metry(LC-IT-TOF-MS), with 18 and 55 metabolites identified, respectively. On this basis, with the help of the obtained accurate molecular weight, characteristic fragment ions, reference comparison, combined with LTC database and natural products database self-created in our group, 18 prototypes and 106 metabolites were tentatively identified in rat plasma after oral gavage of LTC at a dose of 500 mg·kg~(-1). Glucuronidation, sulfonation, and methylation were major biotransformation pathways of LTC. This study preliminarily clarified the LTC constituents absorbed into blood and laid the foundation for clarifying the effective substances of LTC.
Key words
Full text:
1
Database:
WPRIM
Main subject:
Capsules
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Drugs, Chinese Herbal
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Administration, Oral
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Chromatography, High Pressure Liquid
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Chromatography, Liquid
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Gas Chromatography-Mass Spectrometry
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2021
Document type:
Article