Effect of Vitamin D3 to Platelet Activation Mediated by Tumor Cell Culture Medium / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1289-1294, 2021.
Article
in Zh
| WPRIM
| ID: wpr-888554
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the effect of vitamin D3 to platelet activation by tumor cell culture medium.@*METHODS@#The peripheral blood platelets of BALB/c mice were isolated. The platelets were activated in 4T1 culture fluid for 24 h. The platelets were divided into 7 groups control group, activation group, 1 nmol/L vitamin D3 group, 10 nmol/L vitamin D3 group, 50 nmol/L vitamin D3 group, 100 nmol/L vitamin D3 group, and positive drug (0.1 μmol/L eptifibatide) group. CCK-8 assay was used to detect the platelet proliferation at 24, 48 and 72 h. Flow cytometry was used to detect the expression of CD61 and CD62p and receptor for advanced glycation end products (RAGE) at 24, 48 and 72 h. ELISA was used to detect the level of platelet-endothelial cell adhesion molecule-1 (PECAM-1) at 24, 48 and 72 h.@*RESULTS@#The CD41@*CONCLUSION@#Vitamin D3 shows antiplatelet effect and can inhibit platelet proliferation and activation.
Full text:
1
Database:
WPRIM
Main subject:
Blood Platelets
/
Platelet Activation
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Cholecalciferol
/
P-Selectin
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Cell Culture Techniques
/
Flow Cytometry
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Mice, Inbred BALB C
Limits:
Animals
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2021
Document type:
Article