circ_0003204 regulates the osteogenic differentiation of human adipose-derived stem cells via miR-370-3p/HDAC4 axis / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 30-30, 2022.
Article
in En
| WPRIM
| ID: wpr-939849
Responsible library:
WPRO
ABSTRACT
Human adipose-derived stem cells (hASCs) are a promising cell type for bone tissue regeneration. Circular RNAs (circRNAs) have been shown to play a critical role in regulating various cell differentiation and involve in mesenchymal stem cell osteogenesis. However, how circRNAs regulate hASCs in osteogenesis is still unclear. Herein, we found circ_0003204 was significantly downregulated during osteogenic differentiation of hASCs. Knockdown of circ_0003204 by siRNA or overexpression by lentivirus confirmed circ_0003204 could negatively regulate the osteogenic differentiation of hASCs. We performed dual-luciferase reporting assay and rescue experiments to verify circ_0003204 regulated osteogenic differentiation via sponging miR-370-3p. We predicted and confirmed that miR-370-3p had targets in the 3'-UTR of HDAC4 mRNA. The following rescue experiments indicated that circ_0003204 regulated the osteogenic differentiation of hASCs via miR-370-3p/HDAC4 axis. Subsequent in vivo experiments showed the silencing of circ_0003204 increased the bone formation and promoted the expression of osteogenic-related proteins in a mouse bone defect model, while overexpression of circ_0003204 inhibited bone defect repair. Our findings indicated that circ_0003204 might be a promising target to promote the efficacy of hASCs in repairing bone defects.
Full text:
1
Database:
WPRIM
Main subject:
Osteogenesis
/
Repressor Proteins
/
Stem Cells
/
Signal Transduction
/
Cell Differentiation
/
Cells, Cultured
/
Adipose Tissue
/
MicroRNAs
/
RNA, Circular
/
Histone Deacetylases
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
International Journal of Oral Science
Year:
2022
Document type:
Article