Extract human DNA from maggot crop contents by phenol-chloroform method coupled with paramagnetic particle method / 法医学杂志
Journal of Forensic Medicine
; (6): 421-424, 2009.
Article
in Zh
| WPRIM
| ID: wpr-983516
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish an effective phenol-chloroform method coupled with paramagnetic particle method for human DNA extraction from maggot crop contents in STR genotyping.@*METHODS@#Human DNA was extracted from the maggot crop contents using phenol-chloroform method and purified by paramagnetic particle method. DNA was quantified by PCR with Quantifiler Human DNA Quantification Kit using 7500 real-time fluorescence quantitative PCR instrument. PCR products were genotyped by AmpFlSTR Identifiler PCR Amplification Kit using 3130XL-Avant genetic analyzer.@*RESULTS@#The template DNA yield by the method described were increased at least 2 times than the phenol-chloroform extraction method alone. All of the full 16 STR profiles could be obtained with the samples extracted by this method when the DNA yield reached (0.218 +/- 0.041) ng/microL.@*CONCLUSION@#Phenol-chloroform method coupled with paramagnetic particle method can effectively increase the sensitivity of STR analysis of human DNA recovered from maggot crop contents and is a valuable tool for forensic entomology.
Full text:
1
Database:
WPRIM
Main subject:
Cadaver
/
DNA
/
Chloroform
/
Polymerase Chain Reaction
/
DNA Fingerprinting
/
Sensitivity and Specificity
/
Phenol
/
Tandem Repeat Sequences
/
Forensic Sciences
/
Diptera
Limits:
Animals
/
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2009
Document type:
Article