Development of a Forensic Multiplex Amplification STR Kit for 15 Autosomal STR Loci and 10 Y-STR Loci / 法医学杂志
Journal of Forensic Medicine
; (6): 373-380, 2015.
Article
in Zh
| WPRIM
| ID: wpr-984016
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice.@*METHODS@#Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed.@*RESULTS@#A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin.@*CONCLUSION@#The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.
Full text:
1
Database:
WPRIM
Main subject:
DNA Primers
/
Microsatellite Repeats
/
Databases, Nucleic Acid
/
Chromosomes, Human, Y
/
Amelogenin
/
Forensic Genetics
/
Genotyping Techniques
/
Multiplex Polymerase Chain Reaction
/
Genotype
/
Indicators and Reagents
Limits:
Humans
Language:
Zh
Journal:
Journal of Forensic Medicine
Year:
2015
Document type:
Article