Your browser doesn't support javascript.
loading
Characteristic profile of antibody responses to PPD, ESAT-6, and CFP-10 of Mycobacterium tuberculosis in pulmonary tuberculosis suspected cases in Surabaya, Indonesia
Dewi, Desak Nyoman Surya Suameitria; Mertaniasih, Ni Made; Soedarsono,; Ozeki, Yuriko; Artama, Wayan Tunas; Fihiruddin,; Niki, Mamiko; Tateishi, Yoshitaka; Ato, Manabu; Matsumoto, Sohkichi.
Afiliación
  • Dewi, Desak Nyoman Surya Suameitria; Universitas Airlangga. Faculty of Medicine. Doctoral Program of Medical Science. Surabaya. ID
  • Mertaniasih, Ni Made; Universitas Airlangga. Faculty of Medicine. Department of Medical Microbiology. Surabaya. ID
  • Soedarsono,; Universitas Airlangga. Faculty of Medicine. Department of Pulmonology and Respiratory Medicine. Surabaya. ID
  • Ozeki, Yuriko; Niigata University. School of Medicine. Department of Bacteriology. Niigata. JP
  • Artama, Wayan Tunas; Universitas Gadjah Mada. Faculty of Veterinary Medicine. Department of Biochemistry. Yogyakarta. ID
  • Fihiruddin,; Universitas Gadjah Mada. Doctoral Program. Research Center of Biotechnology. Yogyakarta. ID
  • Niki, Mamiko; Osaka City University Graduate. School of Medicine. Department of Bacteriology. Osaka. JP
  • Tateishi, Yoshitaka; Niigata University. School of Medicine. Department of Bacteriology. Niigata. JP
  • Ato, Manabu; National Institute of Infectious Diseases. Leprosy Research Center. Department of Mycobacteriology. Tokyo. JP
  • Matsumoto, Sohkichi; Niigata University. School of Medicine. Department of Bacteriology. Niigata. JP
Braz. j. infect. dis ; 23(4): 246-253, July-Aug. 2019. tab, graf
Artículo en Inglés | LILACS | ID: biblio-1039236
Biblioteca responsable: BR1.1
ABSTRACT
Abstract Accurate and rapid diagnostic tools are important aspects of managing tuberculosis (TB) cases appropriately. However, the sensitivity and specificity of diagnostic kits based on immune response such as the tuberculin skin test (TST) and interferon gamma release assay (IGRA) are still debated. Thus, the exploration and assessment of specific biomarker-targeted antibodies are needed for the development of an accurate and rapid diagnostic tool. The present study was conducted in patients with a respiratory problem suspected to be TB at Dr. Soetomo Hospital, Surabaya, Indonesia. Among 102 patients tested by GeneXpert and AFB, 59 serum samples were from cases retrospectively determined to have active TB. A total of 102 serum of healthy controls (HC) was also collected. The PPD antigen and the recombinant CFP-10 and ESAT-6 proteins were prepared. Antibody responses against these proteins were evaluated by ELISA. All samples were also screened for the possibility of Mycobacterium avium-intracellulare complex (MAC) infection using Capilla MaC kit. The results showed that TB patients had a significantly higher concentration of IgG antibody in response to PPD than the HC. In addition, the receiver operating characteristic (ROC) curve analysis showed that PPD was acceptable for diagnostic purposes with an AUC value of 0.835 (95% CI 0.770-0.900, p < 0.0001). However, ESAT-6 and CFP-10 had low AUCs, and 32 samples from both groups showed a low concentration of IgA antibody against all antigens. The MAC detection results also showed that the concentration of IgA in the HC group was the highest. The current results indicate that PPD is a better antigen for antibody-based detection of TB than ESAT-6 and CFP-10. Based on the MAC detection assay, 53 people in the HC group were probably infected with rapidly growing nontuberculous mycobacteria (NTM), although antibody response to PPD was low.
Asunto(s)


Texto completo: Disponible Colección: Bases de datos internacionales Contexto en salud: ODS3 - Salud y Bienestar / Enfermedades Desatendidas Problema de salud: Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles / Enfermedades Desatendidas / Tuberculosis Base de datos: LILACS Asunto principal: Proteínas Bacterianas / Tuberculina / Tuberculosis Pulmonar / Formación de Anticuerpos / Mycobacterium tuberculosis / Antígenos Bacterianos Tipo de estudio: Estudio diagnóstico / Estudio observacional / Estudio pronóstico Límite: Adolescente / Adulto / Anciano / Niño / Femenino / Humanos / Masculino País/Región como asunto: Asia Idioma: Inglés Revista: Braz. j. infect. dis Asunto de la revista: Enfermedades Transmisibles Año: 2019 Tipo del documento: Artículo País de afiliación: Indonesia / Japón Institución/País de afiliación: National Institute of Infectious Diseases/JP / Niigata University/JP / Osaka City University Graduate/JP / Universitas Airlangga/ID / Universitas Gadjah Mada/ID

Texto completo: Disponible Colección: Bases de datos internacionales Contexto en salud: ODS3 - Salud y Bienestar / Enfermedades Desatendidas Problema de salud: Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles / Enfermedades Desatendidas / Tuberculosis Base de datos: LILACS Asunto principal: Proteínas Bacterianas / Tuberculina / Tuberculosis Pulmonar / Formación de Anticuerpos / Mycobacterium tuberculosis / Antígenos Bacterianos Tipo de estudio: Estudio diagnóstico / Estudio observacional / Estudio pronóstico Límite: Adolescente / Adulto / Anciano / Niño / Femenino / Humanos / Masculino País/Región como asunto: Asia Idioma: Inglés Revista: Braz. j. infect. dis Asunto de la revista: Enfermedades Transmisibles Año: 2019 Tipo del documento: Artículo País de afiliación: Indonesia / Japón Institución/País de afiliación: National Institute of Infectious Diseases/JP / Niigata University/JP / Osaka City University Graduate/JP / Universitas Airlangga/ID / Universitas Gadjah Mada/ID
...