Myelination key factor krox20 is downregulated in Schwann cells and murine sciatic nerves infected by Mycobacterium leprae
s.l; s.n; 2019. 10 p.
Non-conventional
en En
| CONASS, SES-SP, HANSEN, HANSENIASE, SESSP-ILSLPROD, SES-SP, SESSP-ILSLACERVO, SES-SP
| ID: biblio-1147177
Biblioteca responsable:
BR191.1
Ubicación: BR191.1; 9793/S
ABSTRACT
Schwann cells (SCs) critically maintain the plasticity of the peripheral nervous system. Peripheral nerve injuries and infections stimulate SCs in order to retrieve homeostasis in neural tissues. Previous studies indicate that Mycobacterium leprae (ML) regulates the expression of key factors related to SC identity, suggesting that alterations in cell phenotype may be involved in the pathogenesis of neural damage in leprosy. To better understand whether ML restricts the plasticity of peripheral nerves, the present study sought to determine the expression of Krox20, Sox10, cJun and p75NTR in SC culture and mice sciatic nerves, both infected by ML Thai53 strain. Primary SC cultures were stimulated with two different multiplicities of infection (MOI 1001; MOI 501) and assessed after 7 and 14 days. Sciatic nerves of nude mice (NUFoxn1nu) infected with ML were evaluated after 6 and 9 months. In vitro results demonstrate downregulation of Krox20 and Sox10 along with the increase in p75NTRimmunolabelled cells. Concurrently, sciatic nerves of infected mice showed a significant decrease in Krox20 and increase in p75NTR. Our results corroborate previous findings on the interference of ML in the expression of factors involved in cell maturation, favouring the maintenance of a nonmyelinating phenotype in SCs, with possible implications for the repair of adult peripheral nerves(AU).
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Colección:
06-national
/
BR
Base de datos:
CONASS
/
HANSEN
/
HANSENIASE
/
SES-SP
/
SESSP-ILSLACERVO
/
SESSP-ILSLPROD
Asunto principal:
Células de Schwann
/
Lepra
/
Mycobacterium leprae
Límite:
Animals
Idioma:
En
Año:
2019
Tipo del documento:
Non-conventional