Evaluation of the Genotoxicity of Endodontic Materials for Deciduous Teeth Using the Comet Assay
Pesqui. bras. odontopediatria clín. integr
; 21: e0060, 2021. tab
Article
en En
| LILACS, BBO
| ID: biblio-1250451
Biblioteca responsable:
BR1264.1
ABSTRACT
ABSTRACT Objective:
To evaluate genotoxicity of zinc oxide, P. A. calcium hydroxide, mineral trioxide aggregate and an iodoform paste using comet assay on human lymphocytes. Material andMethods:
Two positive controls were used methyl-methanesulfonate for the P.A. calcium hydroxide and mineral trioxide aggregate; and doxorubicin for the iodoform paste and zinc oxide. There were also two negative controls distilled water for the P.A. calcium hydroxide and mineral trioxide aggregate; and DMSO for the iodoform paste and zinc oxide. Comets were identified using fluorescence microscopy and 100 of them were counted on each of the three slides analyzed per drug test. A damage index was established, taking into consideration the score pattern that had previously been determined from the size and intensity of the comet tail. Analysis of variance, followed by Tukey's test, was used to compare the means of the DNA damage indices.Results:
The DNA damage index observed for mineral trioxide aggregate (7.08 to 8.58) and P.A. calcium hydroxide (6.50 to 8.33), which were similar to negative control index. On the other hand, damage index for zinc oxide (104.7 to 218.50) and iodoform paste (115.7 to 210.7) were similar to positive control index.Conclusion:
Iodoform paste and zinc oxide showed genotoxicity at all concentrations used.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
BBO
/
LILACS
Asunto principal:
Diente Primario
/
Óxido de Zinc
/
Ensayo Cometa
/
Genotoxicidad
/
Pruebas de Mutagenicidad
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
ODONTOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Brasil