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Gut dysbiosis, inflammation and type 2 diabetes in mice using synthetic gut microbiota from diabetic humans / Disbiose intestinal, inflamação e diabetes tipo 2 em camundongos usando microbiota intestinal sintética de humanos diabéticos
Liaqat, I; Ali, N M; Arshad, N; Sajjad, S; Rashid, F; Hanif, U; Ara, C; Ulfat, M; Andleeb, S; Awan, U F; Bibi, A; Mubin, M; Ali, S; Tahir, H M; ul-Haq, I.
Afiliación
  • Liaqat, I; GC University Lahore. Department of Zoology. Microbiology Laboratory. Lahore. PK
  • Ali, N M; GC University Lahore. Department of Zoology. Microbiology Laboratory. Lahore. PK
  • Arshad, N; The University of Lahore. Department of Zoology. Lahore. PK
  • Sajjad, S; Lahore College for Women University. Department of Zoology. Lahore. PK
  • Rashid, F; Lahore College for Women University. Department of Zoology. Lahore. PK
  • Hanif, U; GC University. Department of Botany. Lahore. PK
  • Ara, C; University of the Punjab. Department of Zoology. Lahore. PK
  • Ulfat, M; Lahore College for Women University. Department of Botany. Lahore. PK
  • Andleeb, S; University of Azad Jammu and Kashmir. Department of Zoology. Muzaffarabad. PK
  • Awan, U F; GC University. Department of Botany. Lahore. PK
  • Bibi, A; The Women University. Department of Zoology. Multan. PK
  • Mubin, M; University of Agriculture. Centre of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
  • Ali, S; GC University Lahore. Department of Zoology. Microbiology Laboratory. Lahore. PK
  • Tahir, H M; GC University Lahore. Department of Zoology. Microbiology Laboratory. Lahore. PK
  • ul-Haq, I; GC University. Institute of Industrial Biotechnology. Lahore. PK
Braz. j. biol ; 83: e242818, 2023. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1285628
Biblioteca responsable: BR1.1
ABSTRACT
Abstract The study was aimed to assess impact of high fat diet (HFD) and synthetic human gut microbiota (GM) combined with HFD and chow diet (CD) in inducing type-2 diabetes (T2D) using mice model. To our knowledge, this is the first study using selected human GM transplantation via culture based method coupled dietary modulation in mice for in vivo establishment of inflammation leading to T2D and gut dysbiosis. Twenty bacteria (T2D1-T2D20) from stool samples of confirmed T2D subjects were found to be morphologically different and subjected to purification on different media both aerobically and anerobically, which revealed seven bacteria more common among 20 isolates on the basis of biochemical characterization. On the basis of 16S rRNA gene sequencing, these seven isolates were identified as Bacteroides stercoris (MT152636), Lactobacillus acidophilus (MT152637), Lactobacillus salivarius (MT152638), Ruminococcus bromii (MT152639), Klebsiella aerogenes (MT152640), Bacteroides fragilis (MT152909), Clostridium botulinum (MT152910). The seven isolates were subsequently used as synthetic gut microbiome (GM) for their role in inducing T2D in mice. Inbred strains of albino mice were divided into four groups and were fed with CD, HFD, GM+HFD and GM+CD. Mice receiving HFD and GM+modified diet (CD/HFD) showed highly significant (P<0.05) increase in weight and blood glucose concentration as well as elevated level of inflammatory cytokines (TNF-α, IL-6, and MCP-1) compared to mice receiving CD only. The 16S rRNA gene sequencing of 11 fecal bacteria obtained from three randomly selected animals from each group revealed gut dysbiosis in animals receiving GM. Bacterial strains including Bacteroides gallinarum (MT152630), Ruminococcus bromii (MT152631), Lactobacillus acidophilus (MT152632), Parabacteroides gordonii (MT152633), Prevotella copri (MT152634) and Lactobacillus gasseri (MT152635) were isolated from mice treated with GM+modified diet (HFD/CD) compared to strains Akkermansia muciniphila (MT152625), Bacteriodes sp. (MT152626), Bacteroides faecis (MT152627), Bacteroides vulgatus (MT152628), Lactobacillus plantarum (MT152629) which were isolated from mice receiving CD/HFD. In conclusion, these findings suggest that constitution of GM and diet plays significant role in inflammation leading to onset or/and possibly progression of T2D. .
RESUMO
Resumo O estudo teve como objetivo avaliar o impacto da dieta rica em gordura (HFD) e da microbiota intestinal humana sintética (GM) combinada com HFD e dieta alimentar (CD) na indução de diabetes tipo 2 (T2D) usando modelo de camundongos. Para nosso conhecimento, este é o primeiro estudo usando transplante de GM humano selecionado através do método baseado em cultura acoplada à modulação dietética em camundongos para o estabelecimento in vivo de inflamação que leva a T2D e disbiose intestinal. Vinte bactérias (T2D1-T2D20) de amostras de fezes de indivíduos T2D confirmados verificaram ser morfologicamente diferentes e foram submetidas à purificação em meios diferentes aerobicamente e anaerobicamente, o que revelou sete bactérias mais comuns entre 20 isolados com base na caracterização bioquímica. Com base no sequenciamento do gene 16S rRNA, esses sete isolados foram identificados como Bacteroides stercoris (MT152636), Lactobacillus acidophilus (MT152637), Lactobacillus salivarius (MT152638), Ruminococcus bromii (MT152639), Klebsiella aerogenides (MT152640), Bacteroides fragilis (MT152909), Clostridium botulinum (MT152910). Esses sete isolados foram, posteriormente, usados ​​como microbioma intestinal sintético (GM) por seu papel na indução de T2D em camundongos. Linhagens consanguíneas de camundongos albinos foram divididas em quatro grupos e foram alimentadas com CD, HFD, GM + HFD e GM + CD. Camundongos que receberam a dieta modificada com HFD e GM + (CD / HFD) mostraram um aumento altamente significativo (P < 0,05) no peso e na concentração de glicose no sangue, bem como um nível elevado de citocinas inflamatórias (TNF-α, IL-6 e MCP-1) em comparação com os ratos que receberam apenas CD. O sequenciamento do gene 16S rRNA de 11 bactérias fecais obtidas de três animais selecionados aleatoriamente de cada grupo revelou disbiose intestinal em animais que receberam GM. Cepas bacterianas, incluindo Bacteroides gallinarum (MT152630), Ruminococcus bromii (MT152631), Lactobacillus acidophilus (MT152632), Parabacteroides gordonii (MT152633), Prevotella copri (MT152634) e Lactobacillus Gasseri (MT152635D), foram tratadas com dieta modificada / CD) em comparação com as linhagens Akkermansia muciniphila (MT152625), Bacteriodes sp. (MT152626), Bacteroides faecis (MT152627), Bacteroides vulgatus (MT152628), Lactobacillus plantarum (MT152629), que foram isoladas de camundongos recebendo CD / HFD. Em conclusão, esses resultados sugerem que a constituição de GM e dieta desempenham papel significativo na inflamação levando ao início ou/e possivelmente à progressão de T2D.
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Texto completo: Disponible Contexto en salud: Enfermedades Desatendidas Problema de salud: Zoonosis Base de datos: LILACS / VETINDEX Asunto principal: Diabetes Mellitus Tipo 2 / Microbioma Gastrointestinal Tipo de estudio: Ensayo clínico controlado / Estudio pronóstico Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. biol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: GC University Lahore/PK / GC University/PK / Lahore College for Women University/PK / The University of Lahore/PK / The Women University/PK / University of Agriculture/PK / University of Azad Jammu and Kashmir/PK / University of the Punjab/PK

Texto completo: Disponible Contexto en salud: Enfermedades Desatendidas Problema de salud: Zoonosis Base de datos: LILACS / VETINDEX Asunto principal: Diabetes Mellitus Tipo 2 / Microbioma Gastrointestinal Tipo de estudio: Ensayo clínico controlado / Estudio pronóstico Límite: Animales / Humanos Idioma: Inglés Revista: Braz. j. biol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: GC University Lahore/PK / GC University/PK / Lahore College for Women University/PK / The University of Lahore/PK / The Women University/PK / University of Agriculture/PK / University of Azad Jammu and Kashmir/PK / University of the Punjab/PK
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