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Molecular Phylogenetics of Physa acuta (Pulmonata: Basommatophora): an Invasive species in Central Punjab Pakistan / Molecular Phylogenetics of Physa acuta (Pulmonata: Basommatophora): uma espécie invasora no centro de Punjab, Paquistão
Ansari, B; Altafa, J; Ramzan, A; Ahmed, Z; Khalil, S; Qamar, S U R; Awan, S A; Jehangir, K; Khalid, R; Aziz, S; Sultana, T; Sultana, S; Alsamadany, H; Alshamrani, R; Awan, F S.
Afiliación
  • Ansari, B; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Altafa, J; s.af
  • Ramzan, A; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Ahmed, Z; University of Agriculture. Center for Advanced Studies in Agriculture and Food Security. Department of Plant Breeding and Genetics. Faisalabad. PK
  • Khalil, S; The Islamia University of Bahawalpur. Faculty of Agriculture & Environmental Science. Department Forestry Range & Wildlife Management. Bahawalpur. PK
  • Qamar, S U R; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Awan, S A; University of Agriculture. Department of Computer Science. Faisalabad. PK
  • Jehangir, K; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Khalid, R; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Aziz, S; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Sultana, T; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Sultana, S; Government College University Faisalabad. Department of Zoology. Punjab. PK
  • Alsamadany, H; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Alshamrani, R; King Abdulaziz University. Faculty of Science. Department of Biological Sciences. Jeddah. SA
  • Awan, F S; University of Agriculture. Center of Agricultural Biochemistry and Biotechnology. Faisalabad. PK
Braz. j. biol ; 83: e246984, 2023. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1285632
Biblioteca responsable: BR1.1
ABSTRACT
Abstract Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.
RESUMO
Resumo Os físidos pertencem à classe Gastropoda; pertencem ao filo Mollusca e, sendo bioindicadores, hospedeiros intermediários de parasitas e pragas, ocupam uma posição-chave no ecossistema. Existem três espécies do gênero Physa, ou seja, P. fontinalis, Physa acuta e P. gyrina em corpos d'água do Punjab Central e foram caracterizadas com base em marcadores moleculares. Alto nível de diversidade genética foi revelado por RAPD polimórfico, no entanto os marcadores SSR não foram amplificados. A análise multivariada revelou polimorfismo variando de 9,09% a 50% entre as três espécies de Physid. Um número total de 79 loci foi observado para as três espécies em estudo e 24 loci foram observados como polimórficos. Esses fragmentos RAPD podem ser desenvolvidos em marcadores codominantes (SCAR) por clonagem e podem ser posteriormente sequenciados para o desenvolvimento de marcadores específicos da espécie Physa para identificar as espécies introduzidas e nativas no Paquistão.
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Texto completo: Disponible Base de datos: LILACS / VETINDEX Asunto principal: Gastrópodos / Especies Introducidas Límite: Animales País/Región como asunto: Asia Idioma: Inglés Revista: Braz. j. biol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: Government College University Faisalabad/PK / King Abdulaziz University/SA / The Islamia University of Bahawalpur/PK / University of Agriculture/PK
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Texto completo: Disponible Base de datos: LILACS / VETINDEX Asunto principal: Gastrópodos / Especies Introducidas Límite: Animales País/Región como asunto: Asia Idioma: Inglés Revista: Braz. j. biol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: Government College University Faisalabad/PK / King Abdulaziz University/SA / The Islamia University of Bahawalpur/PK / University of Agriculture/PK