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Up-regulation of GINS1 highlighted a good diagnostic and prognostic potential of survival in three different subtypes of human cancer / A regulação positiva de GINS1 destacou um bom potencial diagnóstico e prognóstico de sobrevivência em três subtipos diferentes de câncer humano
Ahmad, M; Hameed, Y; Khan, M; Usman, M; Rehman, A; Abid, U; Asif, R; Ahmed, H; Hussain, M S; Rehman, J U; Asif, H M; Arshad, R; Atif, M; Hadi, A; Sarfraz, U; Khurshid, U.
Afiliación
  • Ahmad, M; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Hameed, Y; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Khan, M; The Islamia University of Bahawalpur. Department of Pharmacy. Bahawalpur. PK
  • Usman, M; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Rehman, A; Qarshi University. Department of Eastern Medicine. Lahore. PK
  • Abid, U; Bahauddin Zakariya University. Department of Pharmaceutics. Multan. PK
  • Asif, R; Government College University Faisalabad. Department of Microbiology. Faisalabad. PK
  • Ahmed, H; Government College University Faisalabad. Department of Eastern Medicine. Faisalabad. PK
  • Hussain, M S; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Rehman, J U; The Islamia University of Bahawalpur. College of Conventional Medicine. Faculty of Pharmacy and Alternative Medicine. Bahawalpur. PK
  • Asif, H M; The Islamia University of Bahawalpur. College of Conventional Medicine. Faculty of Pharmacy and Alternative Medicine. Bahawalpur. PK
  • Arshad, R; The Islamia University of Bahawalpur. College of Conventional Medicine. Faculty of Pharmacy and Alternative Medicine. Bahawalpur. PK
  • Atif, M; The Islamia University of Bahawalpur. College of Conventional Medicine. Faculty of Pharmacy and Alternative Medicine. Bahawalpur. PK
  • Hadi, A; The Islamia University of Bahawalpur. Department of Biochemistry and Biotechnology. Bahawalpur. PK
  • Sarfraz, U; COMSATS University Islamabad. Department of Biosciences. Islamabad. PK
  • Khurshid, U; The Islamia University of Bahawalpur. Department of Pharmacy. Bahawalpur. PK
Braz. j. biol ; 84: e250575, 2024. tab, graf
Artículo en Inglés | LILACS, VETINDEX | ID: biblio-1350309
Biblioteca responsable: BR1.1
ABSTRACT
Abstract Cancer is a fatal malignancy and its increasing worldwide prevalence demands the discovery of more sensitive and reliable molecular biomarkers. To investigate the GINS1 expression level and its prognostic value in distinct human cancers using a series of multi-layered in silico approach may help to establish it as a potential shared diagnostic and prognostic biomarker of different cancer subtypes. The GINS1 mRNA, protein expression, and promoter methylation were analyzed using UALCAN and Human Protein Atlas (HPA), while mRNA expression was further validated via GENT2. The potential prognostic values of GINS1 were evaluated through KM plotter. Then, cBioPortal was utilized to examine the GINS1-related genetic mutations and copy number variations (CNVs), while pathway enrichment analysis was performed using DAVID. Moreover, a correlational analysis between GINS1 expression and CD8+ T immune cells and a the construction of gene-drug interaction network was performed using TIMER, CDT, and Cytoscape. The GINS1 was found down-regulated in a single subtypes of human cancer while commonly up-regulated in 23 different other subtypes. The up-regulation of GINS1 was significantly correlated with the poor overall survival (OS) of Liver Hepatocellular Carcinoma (LIHC), Lung Adenocarcinoma (LUAD), and Kidney renal clear cell carcinoma (KIRC). The GINS1 was also found up-regulated in LIHC, LUAD, and KIRC patients of different clinicopathological features. Pathways enrichment analysis revealed the involvement of GINS1 in two diverse pathways, while few interesting correlations were also documented between GINS1 expression and its promoter methylation level, CD8+ T immune cells level, and CNVs. Moreover, we also predicted few drugs that could be used in the treatment of LIHC, LUAD, and KIRC by regulating the GINS1 expression. The expression profiling of GINS1 in the current study has suggested it a novel shared diagnostic and prognostic biomarker of LIHC, LUAD, and KIRC.
RESUMO
Resumo O câncer é uma doença maligna fatal e sua crescente prevalência mundial exige a descoberta de biomarcadores moleculares mais sensíveis e confiáveis. Investigar o nível de expressão de GINS1 e seu valor prognóstico em cânceres humanos distintos, usando uma série de abordagens in silico em várias camadas, pode ajudar a estabelecê-lo como um potencial biomarcador de diagnóstico e prognóstico compartilhado de diferentes subtipos de câncer. O mRNA de GINS1, a expressão da proteína e a metilação do promotor foram analisados ​​usando UALCAN e Human Protein Atlas (HPA), enquanto a expressão de mRNA foi posteriormente validada via GENT2. Os valores prognósticos potenciais de GINS1 foram avaliados por meio do plotter KM. Em seguida, o cBioPortal foi utilizado para examinar as mutações genéticas relacionadas ao GINS1 e as variações do número de cópias (CNVs), enquanto a análise de enriquecimento da via foi realizada usando DAVID. Além disso, uma análise correlacional entre a expressão de GINS1 e células imunes T CD8 + e a construção de uma rede de interação gene-droga foi realizada usando TIMER, CDT e Cytoscape. O GINS1 foi encontrado regulado negativamente em um único subtipo de câncer humano, enquanto comumente regulado positivamente em 23 outros subtipos diferentes. A regulação positiva de GINS1 foi significativamente correlacionada com a sobrevida global pobre (OS) de Carcinoma Hepatocelular de Fígado (LIHC), Adenocarcinoma de Pulmão (LUAD) e Carcinoma de Células Claras Renais de Rim (KIRC). O GINS1 também foi encontrado regulado positivamente em pacientes LIHC, LUAD e KIRC de diferentes características clínico-patológicas. A análise de enriquecimento de vias revelou o envolvimento de GINS1 em duas vias diversas, enquanto poucas correlações interessantes também foram documentadas entre a expressão de GINS1 e seu nível de metilação do promotor, nível de células imunes T CD8 + e CNVs. Além disso, também previmos poucos medicamentos que poderiam ser usados ​​no tratamento de LIHC, LUAD e KIRC, regulando a expressão de GINS1. O perfil de expressão de GINS1 no estudo atual sugeriu que é um novo biomarcador de diagnóstico e prognóstico compartilhado de LIHC, LUAD e KIRC.
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Texto completo: Disponible Base de datos: LILACS / VETINDEX Asunto principal: Carcinoma de Células Renales / Neoplasias Renales / Neoplasias Hepáticas Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Factores de riesgo Límite: Humanos Idioma: Inglés Revista: Braz. j. biol Año: 2024 Tipo del documento: Artículo Institución/País de afiliación: Bahauddin Zakariya University/PK / COMSATS University Islamabad/PK / Government College University Faisalabad/PK / Qarshi University/PK / The Islamia University of Bahawalpur/PK

Texto completo: Disponible Base de datos: LILACS / VETINDEX Asunto principal: Carcinoma de Células Renales / Neoplasias Renales / Neoplasias Hepáticas Tipo de estudio: Estudio diagnóstico / Estudio pronóstico / Factores de riesgo Límite: Humanos Idioma: Inglés Revista: Braz. j. biol Año: 2024 Tipo del documento: Artículo Institución/País de afiliación: Bahauddin Zakariya University/PK / COMSATS University Islamabad/PK / Government College University Faisalabad/PK / Qarshi University/PK / The Islamia University of Bahawalpur/PK
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