Use of Chelex-100 for the molecular diagnosis of five animal pathogens / Uso de Chelex-100 para el diagnóstico molecular de cinco patógenos de animales
FAVE, Secc. Cienc. vet. (En línea)
; 20(1): 11-12, ene. 2021. tab, graf
Artículo
en Español
|
LILACS-Express
| LILACS
| ID: biblio-1375455
Biblioteca responsable:
AR1.1
RESUMEN
Abstract Molecular tools have improved conventional veterinary diagnosis. Acid nucleic extraction is a key step for downstream applications. This work aimed to compare the DNA extraction method Chelex-100 resin (M1) with Whatman® cards (M2), phenol-chloroform (M3), or commercial kits (M4), and to determine the most sensitive and inexpensive one for its diagnosis of animal pathogens that, despite their economic or zoonotic relevance, receive little attention. DNA was isolated from urine, organs, semen, blood and intestinal mucous, from the bacteria Leptospira interrogans serovar Pomona Pomona (by M1 and M2), Brucella melitensis (by M1, M3 and M4), and Salmonella ser. Abortusequi (by M1 and M4), and the parasites Leishmania spp. (by M1, M3 and M4), and Eimeria spp. (by M1 and M3), respectively. The sensitivity of each method was assayed by Polymerase Chain Reaction (PCR). The M1 showed similar sensitivity for Salmonella ser. Abortusequi, Leishmania spp., and Eimeria spp., being better for L. interrogans serovar Pomona Pomona and slightly lower for B. melitensis. For the first time, a simple and economic method was successfully employed for extracting DNA from these animal pathogens, especially important in low-resource settings, contributing to the diagnosis of leptospirosis, brucellosis, leishmaniasis, and coccidiosis; as well as to the molecular epidemiology of salmonellosis in stallion from semen samples.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Zoonosis
Base de datos:
LILACS
Tipo de estudio:
Estudio diagnóstico
/
Guía de práctica clínica
Idioma:
Español
Revista:
FAVE, Secc. Cienc. vet. (En línea)
Asunto de la revista:
CriaÆo de Animais Domsticos
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Cruzamento
/
Gado
Año:
2021
Tipo del documento:
Artículo
País de afiliación:
Argentina
Institución/País de afiliación:
IPVET - INTA - CONICET/AR
/
UBA/AR