Indoleamine-2,3-dioxygenase-related anti-inflammatory effects of 3-aminobenzamide and infliximab in experimental colitis
Rev. Assoc. Med. Bras. (1992, Impr.)
; 68(3): 362-366, Mar. 2022. tab, graf
Article
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LILACS-Express
| LILACS
| ID: biblio-1376137
Biblioteca responsable:
BR1.1
ABSTRACT
SUMMARY OBJECTIVE:
This study aimed to investigate the presence of indoleamine-2,3-dioxygenase and bacterial translocation after the administration of 3-aminobenzamide and infliximab in the TNBS model of rat colitis.METHODS:
The study group was divided into five categories as follows group 1 (control), group 2 colitis+saline, group 3 colitis+3-aminobenzamide, group 4 colitis+infliximab, and group 5 colitis+3-aminobenzamide+infliximab. Intestinal mesenteric cultures were incubated on specific agar media plates under aerobic and anaerobic conditions, bacterial translocation was evaluated and assessed as colony-forming units per gram of tissue. Colonic tissue samples were evaluated by Western blotting method to detect the presence of indoleamine-2,3-dioxygenase.RESULTS:
The results obtained were as follows group 1 normal gut flora; group 2 eight of nine samples had bacterial translocation, of which six of them had positive indoleamine-2,3-dioxygenase protein; group 3 five of nine samples had bacterial translocation, of which seven of them had positive indoleamine-2,3-dioxygenase; group 4 three of nine samples had bacterial translocation, of which seven of them had positive indoleamine-2,3-dioxygenase; and group 5 only one sample had exact indoleamine-2,3-dioxygenase protein.CONCLUSION:
Altered expression of indoleamine-2,3-dioxygenase results in a lower bacterial translocation via infliximab compared with 3-aminobenzamide treatment. Combined treatments emphasized different approaches for the new molecules related to indoleamine-2,3-dioxygenase.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Idioma:
En
Revista:
Rev. Assoc. Med. Bras. (1992, Impr.)
Asunto de la revista:
EducaÆo em Sa£de
/
GestÆo do Conhecimento para a Pesquisa em Sa£de
/
MEDICINA
Año:
2022
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Brasil