Molecular characterization of nontuberculous Mycobacteria in a tuberculosis and HIV reference unit in the State of Amazonas, Brazil
Rev. Soc. Bras. Med. Trop
; 55: e0613, 2022. tab
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1387533
Biblioteca responsable:
BR1.1
ABSTRACT
ABSTRACT Background:
In recent years, the prevalence of nontuberculous mycobacterial (NTM) infections has increased in different regions of the world. The American Thoracic Society (ATS) recommends standardized identification criteria, reinforcing the need for faster and less complicated clinical and laboratory techniques.Methods:
In this retrospective study, NTM species isolated from pulmonary, extrapulmonary, and disseminated samples from patients treated at a TB/HIV reference unit in the State of Amazonas from 2011 to 2014 were identified through a combination of molecular techniques.Results:
To identify the molecular technique, 50 cryopreserved NTM cultures were recovered and subcultivated in culture medium. The potentially pathogenic NTM species identified were M. avium, M. intracellulare, M. kansasii, M. chelonae, M. abscessus, M. fortuitum, and M. peregrinum. Results of GenoType® showed moderate agreement with those of genomic sequencing (kappa = 0.60), whereas the results obtained by the PRA-hsp65 technique disagreed with the results obtained by sequencing (kappa = 0.49).Conclusions:
Our findings highlight that GenoType CM is a good method for the identification of NTM, as well as the need for the application of standardized criteria, such as those set forth by the ATS.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Enfermedades Desatendidas
/
Tuberculosis
Base de datos:
LILACS
Tipo de estudio:
Estudio observacional
/
Estudio pronóstico
/
Factores de riesgo
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Rev. Soc. Bras. Med. Trop
Asunto de la revista:
Medicina Tropical
Año:
2022
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Fundação Oswaldo Cruz/BR
/
Fundação de Medicina Tropical Dr. Heitor Vieira Dourado/BR
/
Secretaria de Estado da Saúde do Rio Grande do Sul/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade Federal de Ouro Preto/BR
/
Universidade Federal do Rio de Janeiro/BR
/
Universidade Luterana do Brasil/BR
/
Universidade do Estado do Amazonas/BR