Lineage and phylogenetic analysis of HPV-16, -18 in saliva of HNSCC patients
Braz. j. oral sci
; 21: e226337, jan.-dez. 2022. ilus
Artículo
en Inglés
| LILACS, BBO - Odontología
| ID: biblio-1392920
Biblioteca responsable:
BR218.1
ABSTRACT
Aim:
Head and Neck Squamous Cell Carcinoma (HNSCC) is a global health problem whose incidence varies by geographic region and race according to risk factors. Human papillomavirus (HPV) infection is a significant risk factor for HNSCC. HPV-16 and HPV-18 are two forms of HPV that are carcinogenic. HNSCCs that are HPV positive have a better prognosis rather than HPV negative. The purpose of this research was to characterize HPV-16, -18 variations in the saliva of HNSCC patients by examining the genetic diversity of HPV-16, -18 utilizing the full E6, E7, and L1 genes.Methods:
The case-control research included 15 patients with HNSCC and 15 healthy volunteers. Unstimulated entire saliva samples were obtained from the case and control groups by spitting method. Genomic DNA was isolated from all saliva samples. A PCR reaction was used to determine the presence of HPV in saliva. HPV-positive samples were genotyped and data were analyzed. We conducted a variant study on the HPV-16, -18 E6, and E7 genes.Results:
Three patients with HNSCC were HPV-positive for two HPV genotypes out of 30 people diagnosed with HPV-DNA. HPV-16 and -18 were the most common genotypes. The HPV-16, -18 E6, and E7 genes were sequenced and compared to the HPV-16, -18 (E6, E7) prototype sequence. In all, HPV-16 lineages A1 and HPV-18 lineages A3 were discovered.Conclusion:
Regarding the variation of HPV found in Iranian HNSCC patients, the need for further studies in HPV genotyping was seen. Sequencing HPV genes in HNSCC may help answer questions about HPV genotyping in the Iranian population. HPV genotype analysis aids in the development of vaccinations against HNSCC, halting disease progression and preventing HPV-associated HNSCC
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
ODS3 - Salud y Bienestar
Problema de salud:
Meta 3.8: Alcanzar cobertura universal de salud
Base de datos:
BBO - Odontología
/
LILACS
Asunto principal:
Filogenia
/
Saliva
/
Papillomavirus Humano 16
/
Papillomavirus Humano 18
/
Alphapapillomavirus
/
Carcinoma de Células Escamosas de Cabeza y Cuello
Tipo de estudio:
Estudio pronóstico
/
Factores de riesgo
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Braz. j. oral sci
Asunto de la revista:
Odontología
Año:
2022
Tipo del documento:
Artículo
País de afiliación:
Irán
Institución/País de afiliación:
Razi Vaccine and Serum Research Institute/IR
/
Tehran University of Medical Science/IR
/
Tehran University of Medical Sciences/IR