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Cornuside ameliorated experimental autoimmune encephalomyelitis by limiting the recruitment of CD4+ T lymphocytes in the spinal cord
Zhang, Rongbo; Zhang, Shuijing; Xu, Bin; Wu, You; Liang, Shunli; Hou, Bonan; Wang, Mimi; Liu, Jin; Yuan, Qiang.
Afiliación
  • Zhang, Rongbo; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Zhang, Shuijing; Zhejiang Rehabilitation Medical Center. Department of Neurological Rehabilitation. Hangzhou. CN
  • Xu, Bin; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Wu, You; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Liang, Shunli; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Hou, Bonan; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Neurology. Hangzhou. CN
  • Wang, Mimi; The Second Affiliated Hospital of Zhejiang Chinese Medical University. Department of Pharmacy. Hangzhou. CN
  • Liu, Jin; Zhejiang Chinese Medical University. College of Pharmacy. Hangzhou. CN
  • Yuan, Qiang; Zhejiang Chinese Medical University. College of Pharmacy. Hangzhou. CN
Braz. J. Pharm. Sci. (Online) ; 58: e191070, 2022. tab, graf
Article en En | LILACS | ID: biblio-1394044
Biblioteca responsable: BR40.1
Ubicación: BR40.1
ABSTRACT
We conducted this study to determine whether cornuside could improve the neurological deficit symptoms of experimental autoimmune encephalomyelitis (EAE) rats, as well as determine the potential involvement of CD4+ T lymphocytes, vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and tumor necrosis factor-α (TNF-α). Altogether, 32 Lewis rats were randomly divided into control, EAE, EAE/prednisolone, and EAE/cornuside, wherein their neurological function was assessed every day. CD4+ T lymphocyte recruitment into the spinal cord (SC) was evaluated using immunohistochemistry. The VCAM-1, ICAM-1 and TNF-α mRNA expressions in the SC were determined by real-time quantitative PCR, and the VCAM-1 and ICAM-1 proteins were determined by western blotting. Compared to the control group, the EAE group rats with neurological deficits had enhanced CD4+ T lymphocyte infiltration and higher expression levels of VCAM-1, ICAM-1, and TNF-α in the SC. Meanwhile, compared with the EAE group, the EAE/cornuside and EAE/prednisolone groups had lower neurological scores, less CD4+ T lymphocyte infiltrations, and lower expression levels of VCAM-1, ICAM-1, and TNF-α in the SC. Thus, cornuside ameliorated EAE, which could be owed to the inhibition of CD4+ T lymphocyte recruitment and VCAM-1, ICAM-1, and TNF-α expressions in the SC
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Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Médula Espinal / Linfocitos T CD4-Positivos / Encefalomielitis Autoinmune Experimental Límite: Animals Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Médula Espinal / Linfocitos T CD4-Positivos / Encefalomielitis Autoinmune Experimental Límite: Animals Idioma: En Revista: Braz. J. Pharm. Sci. (Online) Asunto de la revista: Farmacologia / Terapˆutica / Toxicologia Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Brasil