First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil
Mem. Inst. Oswaldo Cruz
; 118: e230071, 2023. tab, graf
Artículo
en Inglés
|
LILACS-Express
| LILACS
| ID: biblio-1514607
Biblioteca responsable:
BR1.1
ABSTRACT
BACKGROUND Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania. OBJECTIVES In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions. METHODS A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene. FINDINGS We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively. MAIN CONCLUSIONS To the best of our knowledge, this is the first detection of LRV2 in the New World.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
LILACS
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
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Parasitología
Año:
2023
Tipo del documento:
Artículo
País de afiliación:
Brasil
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República Checa
Institución/País de afiliación:
Fundação Oswaldo Cruz-Fiocruz/BR
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Ministério da Agricultura, Pecuária e Abastecimento/BR
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Universidade Federal da Bahia/BR
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Universidade Federal de Minas Gerais/BR
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Universidade Federal de São João Del Rei/BR
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Universidade Federal do Paraná/BR
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Universidade de São Paulo/BR
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University of Ostrava/CZ