Salivary Metabolites in Patients with Mucopolysaccharidosis
Pesqui. bras. odontopediatria clín. integr
; 24: e220151, 2024. graf
Artículo
en Inglés
| LILACS, BBO - Odontología
| ID: biblio-1535004
Biblioteca responsable:
BR1264.1
ABSTRACT
ABSTRACT Objective:
To identify the salivary metabolites profile of Mucopolysaccharidosis (MPS) types I, II, IV, and VI patients. Material andMethods:
The participants were asked to refrain from eating and drinking for one hour before sampling, performed between 730 and 900 a.m. Samples were centrifuged at 10.000 × g for 60 min at 4°C, and the supernatants (500µl) were stored at −80°C until NMR analysis. The salivary proton nuclear magnetic resonance (1H-NMR) spectra were acquired in a 500 MHz spectrometer, and TOCSY experiments were used to confirm and assign metabolites. Data were analyzed descriptively.Results:
Differences in salivary metabolites were found among MPS types and the control, such as lactate, propionate, alanine, and N-acetyl sugar. Understanding these metabolite changes may contribute to precision medicine and early detection of mucopolysaccharidosis and its monitoring.Conclusion:
The composition of low molecular weight salivary metabolites of mucopolysaccharidosis subjects may present specific features compared to healthy controls.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Base de datos:
BBO - Odontología
/
LILACS
Asunto principal:
Saliva
/
Espectroscopía de Resonancia Magnética
/
Mucopolisacaridosis
/
Metabolómica
/
Espectroscopía de Protones por Resonancia Magnética
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Inglés
Revista:
Pesqui. bras. odontopediatria clín. integr
Asunto de la revista:
Odontología
Año:
2024
Tipo del documento:
Artículo
País de afiliación:
Brasil
Institución/País de afiliación:
Federal University of Rio de Janeiro/BR
/
State University of Rio de Janeiro/BR