Cryopreservation of banana's cv Grand Naine in vitro rhizomes
An. acad. bras. ciênc
; 90(1): 495-507, Mar. 2018. tab, graf
Article
en En
| LILACS
| ID: biblio-886920
Biblioteca responsable:
BR1.1
ABSTRACT
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Floroglucinol
/
Criopreservación
/
Crioprotectores
/
Musa
/
Rizoma
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
An. acad. bras. ciênc
Asunto de la revista:
CIENCIA
Año:
2018
Tipo del documento:
Article
País de afiliación:
Brasil