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Correlation between the BACTEC MGIT 960 culture system with Genotype MTBDRplus and TB-SPRINT in multidrug resistant Mycobacterium tuberculosis clinical isolates from Brazil
Dantas, Nayanne Gama Teixeira; Suffys, Phillip Noel; Carvalho, Wânia da Silva; Gomes, Harrison Magdinier; Almeida, Isabela Neves de; Figueiredo, Lida Jouca de Assis; Gonçalves, Alan Douglas; Gomgnimbou, Michel Kireopori; Refregier, Guislaine; Sola, Christophe; Miranda, Silvana Spíndola de.
Afiliación
  • Dantas, Nayanne Gama Teixeira; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Suffys, Phillip Noel; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Carvalho, Wânia da Silva; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Gomes, Harrison Magdinier; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Almeida, Isabela Neves de; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Figueiredo, Lida Jouca de Assis; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Gonçalves, Alan Douglas; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Gomgnimbou, Michel Kireopori; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Refregier, Guislaine; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Sola, Christophe; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
  • Miranda, Silvana Spíndola de; Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Clínica Médica. Belo Horizonte. BR
Mem. Inst. Oswaldo Cruz ; 112(11): 769-774, Nov. 2017. tab
Artículo en Inglés | LILACS | ID: biblio-894852
Biblioteca responsable: BR1.1
ABSTRACT
BACKGROUND The accurate detection of multidrug-resistant tuberculosis (MDR-TB) is critical for the application of appropriate patient treatment and prevention of transmission of drug-resistant Mycobacterium tuberculosis isolates. The goal of this study was to evaluate the correlation between phenotypic and molecular techniques for drug-resistant tuberculosis diagnostics. Molecular techniques used were the line probe assay genotype MTBDRplus and the recently described tuberculosis-spoligo-rifampin-isoniazid typing (TB-SPRINT) bead-based assay. Conventional drug susceptibility testing (DST) was done on a BACTECTM MGIT 960 TB. METHOD We studied 80 M. tuberculosis complex (MTC) clinical isolates from Minas Gerais state, of which conventional DST had classified 60 isolates as MDR and 20 as drug susceptible. FINDINGS Among the 60 MDR-TB isolates with MGIT as a reference, sensitivity, specificity, accuracy, and kappa for rifampicin (RIF) resistance using TB-SPRINT and MTBDRplus, were 96.7% versus 93.3%, 100.0% versus 100.0%, 97.5% versus 95.0% and 0.94 versus 0.88, respectively. Similarly, the sensitivity, specificity, accuracy, and kappa for isoniazid (INH) resistance were 85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71 for both tests, respectively. Finally, the sensitivity, specificity, accuracy, and kappa for MDR-TB were 85.0% and 83.3%, 100.0% and 100.0%, 88.8% and 87.5% and 0.74 and 0.71 for both tests, respectively. MAIN CONCLUSIONS Both methods exhibited a good correlation with the conventional DST. We suggest estimating the cost-effectiveness of MTBDRplus and TB-SPRINT in Brazil.
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Texto completo: Disponible Colección: Bases de datos internacionales Contexto: OBJETIVO DE DESARROLLO SOSTENIBLE 3 - ODS3 / Enfermedades desatendidas Tema en salud: Meta 3.3: Poner fin a las enfermedades desatendidas y detener enfermedades transmisibles / Tuberculosis Base de datos: LILACS Asunto principal: Técnicas Bacteriológicas / Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Límite: Humanos País/Región como asunto: America del Sur / Brasil Idioma: Inglés Revista: Mem. Inst. Oswaldo Cruz Asunto de la revista: Medicina Tropical / Parasitología Año: 2017 Tipo del documento: Artículo / Documento de proyecto Institución/País de afiliación: Universidade Federal de Minas Gerais/BR