History, extensive characterization and challenge of anti-tetanus serum from world war I: exciting remnants and deceived hopes
Immunol Res, v. 68, p. 7-12, mar. 2020
Artículo
en Inglés
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: bud-2967
Biblioteca responsable:
BR78.1
ABSTRACT
During World War I (WWI), infectious diseases including tetanus were among the most important causes of death. Even though its efficacy was somewhat controversial before the war, tetanus antiserum played a key role in reducing the mortality of this disease. A vial of tetanus antiserum dating back from WWI, left behind on the French battlefield by the US Army, was borrowed from a private collection and opened. The serum contained within was characterized by orthogonal biochemical techniques to determine if any neutralizing IgGs could remain after 100 years of storage. In vitro analysis by Size Exclusion Chromatography and Serum Protein Electrophoresis suggested the presence of residual IgG. In spite of our hopes, these IgGs were not able to protect mice against tetanus toxin challenge in a neutralizing assay. Even though our results indicate the presence of remaining IgGs inside the serum, they were functionally disabled. These results show that obscurity alone is insufficient to protect IgGs from degradation over very long periods of time at room temperature.
Texto completo:
Disponible
Colección:
Bases de datos nacionales
/
Brasil
Base de datos:
Sec. Est. Saúde SP
/
SESSP-IBPROD
Idioma:
Inglés
Revista:
Immunol Res
Año:
2020
Tipo del documento:
Artículo