Identification and modeling of a novel chloramphenicol resistance protein detected by functional metagenomics in a wetland of Lerma, Mexico
Int. microbiol
; 16(2): 103-111, jun. 2013. ilus, tab
Article
en En
| IBECS
| ID: ibc-126425
Biblioteca responsable:
ES1.1
Ubicación: BNCS
ABSTRACT
The exploration of novel antibiotic resistance determinants in a particular environment may be limited because of the presence of uncultured microorganisms. In this work, a culture independent approach based on functional metagenomics was applied to search for chloramphenicol resistance genes in agro-industrial wastewater in Lerma de Villada, Mexico. To this end, a metagenomic library was generated in Escherichia coli DH10B containing DNA isolated from environmental samples of the residual arsenic-enriched (10 mg/ml) effluent. One resistant clone was detected in this library and further analyzed. An open reading frame similar to a multidrug resistance protein from Aeromonas salmonicida and responsible for chloramphenicol resistance was identifi ed, sequenced, and found to encode a member of the major facilitator superfamily (MFS). Our results also showed that the expression of this gene restored streptomycin sensitivity in E. coli DH10B cells. To gain further insight into the phenotype of this MFS family member, we developed a model of the membrane protein multiporter that, in addition, may serve as a template for developing new antibiotics (AU)
RESUMEN
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Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Resistencia al Cloranfenicol
/
Metagenómica
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
Mexico
Idioma:
En
Revista:
Int. microbiol
Año:
2013
Tipo del documento:
Article