Effect of acid and in vitro digestion on conformation and IgE-binding capacity of major oyster allergen Cra g 1 (tropomyosin)
Allergol. immunopatol
; 48(1): 26-33, ene.-feb. 2020. ilus, graf
Artículo
en Inglés
| IBECS
| ID: ibc-186588
Biblioteca responsable:
ES1.1
Ubicación: BNCS
ABSTRACT
Introduction and Objectives:
The production and consumption of oysters is increasing annually because it can provide essential nutrients and benefit for human health, leading to frequent occurrence of severe allergic reactions observed in sensitized individuals. The aim of the present study was to investigate the effects of acid and protease treatment on the conformation and IgE-binding capacity of recombinant Crassostrea gigas tropomyosin (Cra g 1).Results:
Under acidic conditions, Cra g 1 did not undergo degradation, however, the changes obvious in the intensity of CD signal and ANS-binding fluorescence were observed, which was associated with a decrease in antibody reactivity. In simulated gastrointestinal fluid (SGF) and simulated intestinal fluid (SIF) digestion system, acid-treated Cra g 1 was relatively resistant to digestion, but the degradative patterns were very different. Moreover, owing to alterations of secondary structure and hydrophobic surface of the protein during digestive processing, antigenicity of acid-induced Cra g 1 reduced in SGF while it increased significantly in SIF.Conclusion:
To our knowledge, this is the first study reporting that antigenicity of acid-treated oyster tropomyosin increased after SIF digestion. These results revealed that treatment with acid and pepsin, rather than trypsin, was an effective way of reducing IgE-binding capacity of tropomyosin from oysterRESUMEN
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Colección:
Bases de datos nacionales
/
España
Base de datos:
IBECS
Asunto principal:
Tropomiosina
/
Técnicas In Vitro
/
Inmunoglobulina E
/
Alérgenos
/
Digestión
/
Hipersensibilidad a los Alimentos
Límite:
Humanos
Idioma:
Inglés
Revista:
Allergol. immunopatol
Año:
2020
Tipo del documento:
Artículo
Institución/País de afiliación:
China National Research Institute of Food and Fermentation Industries/China