Clinicopathological and ultrastructural characterization of periapical actinomycosis
Med. oral patol. oral cir. bucal (Internet)
; 25(1): e131-e136, ene. 2020. ilus, tab
Article
en En
| IBECS
| ID: ibc-196205
Biblioteca responsable:
ES1.1
Ubicación: BNCS
ABSTRACT
BACKGROUND:
The aim of the present study was to analyze the clinicopathological and the ultrastructural features of periapical actinomycosis (PA) cases. MATERIAL ANDMETHODS:
Data from the files of an oral pathology laboratory were retrieved and the findings of histopathological analysis were evaluated. Hematoxylin-eosin (HE), a modified Brown & Brenn, and Grocott stains as well as ultrastructural analysis using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were utilized.RESULTS:
Six cases were obtained, 4 females and 2 males, with a mean age of 34 year-old. Two cases were symptomatic, lower teeth and the anterior region were more commonly affected, and all cases were characterized by periapical radiolucencies. All cases presented sulfur granules with a ray-fungus or club-shaped pattern of the Splendore-Hoeppli phenomenon in HE-stained sections, with filamentous gram-positive bacteria aggregates highlighted by the modified Brown & Brenn stain. SEM analysis revealed abundant packed rod-like and filamentous bacteria associated with an extracellular amorphous material. EDX analysis showed predominant picks of calcium and sulfur in actinomycotic colonies.CONCLUSIONS:
Our findings suggest that PA manifests either clinically and radiologically as a non-specific and heterogeneous condition and that the actinomycotic colonies consist in a calcium- and sulfur-rich matrix. Furthermore, the results highlight the importance of submitting periapical specimens after surgical removal to histopathological análisisRESUMEN
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Texto completo:
1
Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Enfermedades Periapicales
/
Actinomicosis
Límite:
Adult
/
Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Med. oral patol. oral cir. bucal (Internet)
Año:
2020
Tipo del documento:
Article