MicroRNA-802 promotes the progression of osteosarcoma through targeting p27 and activating PI3K/AKT pathway
Clin. transl. oncol. (Print)
; 24(2): 266-275, febrero 2022.
Artículo
en Inglés
| IBECS
| ID: ibc-203432
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
PurposeIncreasing evidences suggest dysfunctions of microRNAs (miRNAs) are playing important part in tumors. Therefore, the role of miR-802 in osteosarcoma (OS) was exploited. The object was to evaluate the effect of miR-802 and verify its influence on p27 Kip1 (p27) in OS.MethodsRT-qPCR experiment was used to detect miR-802 and p27 expression in OS tissues and cells. We explored the function of miR-802 through Transwell assays. The phosphoinositide 3-kinase (PI3K)/AKT serine/threonine kinase pathway and epithelialmesenchymal transition (EMT) was detected by Western blot assays. Luciferase assay was used to testify the target of miR-802.ResultsMiR-802 expression was elevated in OS, which was related to poor clinical outcome in OS patients. MiR-802 overexpression promoted OS migration, invasion and EMT. Further, p27 is a direct target of miR-802. P27 elevation counteracted the promotion effect of OS on EMT, migration and invasion induced by miR-802. In addition, miR-802 overexpression inactivated PI3K/AKT pathway via targeting p27 in OS.ConclusionMiR-802 promoted the progress of EMT, migration and invasion in OS via targeting p27. This newly identified miR-802/p27/PI3K/AKT axis may represent potential targets for OS.
Texto completo:
Disponible
Colección:
Bases de datos nacionales
/
España
Base de datos:
IBECS
Asunto principal:
Osteosarcoma
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MicroARNs
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Ciencias de la Salud
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Transición Epitelial-Mesenquimal
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Neoplasias
Límite:
Humanos
Idioma:
Inglés
Revista:
Clin. transl. oncol. (Print)
Año:
2022
Tipo del documento:
Artículo
Institución/País de afiliación:
Qingdao Hospital of Traditional Chinese Medicine/China
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Traditional Chinese Medical Hospital of Huangdao District/China
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Weifang Weiyi Tumor Hospital/China
/
Zhangqiu District Peoples Hospital/China