MARCH1 silencing suppresses growth of oral squamous cell carcinoma through regulation of PHLPP2
Clin. transl. oncol. (Print)
; 24(7): 1311-1321, julio 2022. graf
Article
en En
| IBECS
| ID: ibc-203830
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
PurposeOral squamous cell carcinoma (OSCC) is the most frequent type of oral cancer and is associated with high mortality. Membrane-associated ring-CH type finger 1 (MARCH1) is an E3 ubiquitin ligase with roles in immune regulation and cancer development. Whether MARCH1 has a specific role in OSCC, and if so through what mechanism, has not been explored.MethodsImmunohistochemistry was performed to examine MARCH1 expression in OSCC clinical samples and adjacent paracancerous tissues. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot were conducted to determine mRNA expression and protein levels, respectively. Knockdown and overexpression experiments were carried out to evaluate the effects of MARCH1 on proliferation and apoptosis. To test proteinprotein interaction, co-immunoprecipitation assay was performed. Finally, tumor cell grafting was utilized to test the function of MARCH in vivo.ResultsHigh MARCH1 expression in OSCC clinical samples correlated with poor patient prognosis. Functionally, MARCH1 knockdown in OSCC cells suppressed proliferation and promoted apoptosis, while MARCH1 overexpression displayed the opposite effects. We identified PH Domain And Leucine Rich Repeat Protein Phosphatase (PHLPP) 2 as an important target of MARCH1. Mechanistically, MARCH1 interacted with PHLPP2 and promoted PHLPP2 ubiquitination. Lastly, MARCH1 knockdown suppressed OSCC tumorigenicity in vivo and increased PHLPP2 protein level.
Palabras clave
Texto completo:
1
Colección:
06-national
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ES
Base de datos:
IBECS
Asunto principal:
Carcinoma de Células Escamosas
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Movimiento Celular
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Apoptosis
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Fosfoproteínas Fosfatasas
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Línea Celular Tumoral
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Proliferación Celular
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Carcinoma de Células Escamosas de Cabeza y Cuello
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Neoplasias de Cabeza y Cuello
Límite:
Humans
Idioma:
En
Revista:
Clin. transl. oncol. (Print)
Año:
2022
Tipo del documento:
Article