TIPE1 inhibits the growth of Ewings sarcoma cells by suppressing Wnt/B-catenin signaling
Clin. transl. oncol. (Print)
; 25(5): 1332-1339, mayo 2023. graf
Article
en En
| IBECS
| ID: ibc-219517
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Background Ewings sarcoma is the second most common bone and soft tissue malignancy in children and adolescents. Tumor necrosis factor-α-induced protein 8-like 1 (TIPE1) functions as a tumor suppressor in several cancers. Activation of Wnt/β-catenin signaling in subpopulations of tumor cells contributes to phenotypic heterogeneity and disease progression in Ewings sarcoma. The exact role of TIPE1 in Ewings sarcoma remains to be elucidated. Purpose This study aimed to assess the expression and function of TIPE1 in Ewings sarcoma. Method TIPE1 expression in Ewings sarcoma cells was determined by qPCR and western blotting. Furthermore, the Ewings sarcoma cell line RD-ES was transfected with a lentivirus-based TIPE1 expression system to upregulate the expression of TIPE1. The Cell Counting Kit 8 was used to assess the effect of TIPE1 on cell proliferation. The effects of TIPE1 on cell migration and invasion was detected by Transwell assay. Flow cytometry was performed to detect apoptosis. Results Our results suggested lower TIPE1 expression in Ewings sarcoma cell lines compared with normal osseous cells. TIPE1 remarkably inhibited the growth and proliferation of Ewings sarcoma cell; TIPE1 also induced apoptosis and inhibited invasion in vitro. TIPE1 inhibited Ewings sarcoma growth, motility, and survival through regulation of Wnt/β-catenin signaling. Conclusions Our results demonstrated the anti-tumor function of TIPE1 in Ewings sarcoma and reveal a novel therapeutic target (AU)
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Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Sarcoma de Ewing
/
Neoplasias Óseas
Límite:
Adolescent
/
Child
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Humans
Idioma:
En
Revista:
Clin. transl. oncol. (Print)
Año:
2023
Tipo del documento:
Article