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MiR-185-5p measurement assists in reflecting Th1/Th2 cell imbalance, inflammatory cytokine production, and exacerbation risk for childhood asthma
Li, Chunmei; Zhao, Yiran; Cai, Chunquan; Liu, Zhenkui; Ma, Junshuai; Song, Yanhong; Zhang, Yanwen; Guo, Weiping; Lu, Yanhui; Xing, Jing.
Afiliación
  • Li, Chunmei; Tianjin Medical University. Tianjin. China
  • Zhao, Yiran; Tianjin Medical University. Tianjin. China
  • Cai, Chunquan; Tianjin Children's Hospital. Tianjin Institute of Pediatrics. Department of Neurosurgery. Tianjin. China
  • Liu, Zhenkui; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Ma, Junshuai; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Song, Yanhong; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Zhang, Yanwen; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Guo, Weiping; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Lu, Yanhui; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
  • Xing, Jing; The First Affiliated Hospital of Hebei North University. Department of Pediatric. Zhangjiakou. China
Allergol. immunopatol ; 51(3): 91-98, 01 mayo 2023. tab
Artículo en Inglés | IBECS | ID: ibc-219817
Biblioteca responsable: ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT

Background:

MicroRNA (miR)-185-5p participates in the pathology of asthma by regulating immune imbalance, inflammation, periostin synthesis, and smooth muscle contraction. This study intended to explore the dysregulation of miR-185p and its correlation with T-helper (Th)1, Th2 cells, and inflammatory cytokines in childhood asthma.

Methods:

In 150 childhood asthma patients and 30 healthy controls (HCs), miR-185-5p from peripheral blood mononuclear cells was detected using reverse transcription-quantitative polymerase chain reaction, Th cells from peripheral blood samples were detected using flow cytometry, inflammatory cytokines from serum samples were detected using enzyme-linked immunosorbent assay.

Results:

MiR-185-5p was increased in childhood asthma patients versus HCs [median (interquartile range (IQR)) 2.315 (1.770–3.855) versus 1.005 (0.655–1.520)] (P < 0.001). Meanwhile, miR-185-5p was negatively associated with Th1 cells (P = 0.035) but positively correlated with Th2 cells (P = 0.006) and IL-4 (P = 0.003) in childhood asthma patients; however, miR-185-5p was not linked to Th1 cells, Th2 cells, IFN-γ, or IL-4 in HCs (all P > 0.05). In addition, miR-185-5p was positively related to TNF-α (P < 0.001), IL-1β (P = 0.015), and IL-6 (P = 0.008) in childhood asthma patients, miR-185-5p was only linked to TNF-α (P = 0.040) but not IL-1β or IL-6 (both P > 0.05) in HCs. Moreover, miR-185-5p was increased in exacerbated childhood asthma patients versus remissive patients [median (IQR) 3.170 (2.070–4.905) versus 1.900 (1.525–2.615)] (P < 0.001). Besides, miR-185-5p was highest in patients with severe exacerbation followed by patients with moderate exacerbation, and lowest in patients with mild exacerbation (P = 0.010).

Conclusion:

MiR-185-5p is associated with imbalanced Th1/Th2 cells, increased inflammatory cytokines along with elevated exacerbation risk, and severity in childhood asthma patients (AU)
Asunto(s)

Texto completo: Disponible Colección: Bases de datos nacionales / España Base de datos: IBECS Asunto principal: Asma / Citocinas / Células Th2 / Células TH1 / Inflamación Límite: Humanos Idioma: Inglés Revista: Allergol. immunopatol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: The First Affiliated Hospital of Hebei North University/China / Tianjin Children's Hospital/China / Tianjin Medical University/China
Texto completo: Disponible Colección: Bases de datos nacionales / España Base de datos: IBECS Asunto principal: Asma / Citocinas / Células Th2 / Células TH1 / Inflamación Límite: Humanos Idioma: Inglés Revista: Allergol. immunopatol Año: 2023 Tipo del documento: Artículo Institución/País de afiliación: The First Affiliated Hospital of Hebei North University/China / Tianjin Children's Hospital/China / Tianjin Medical University/China
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