miR-144-3p increases radiosensibility of gastric cancer cells by targeting inhibition of ZEB1
Clin. transl. oncol. (Print)
; 23(3): 491-500, mar. 2021. graf
Article
en En
| IBECS
| ID: ibc-220884
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Purpose This study set out to probe into the effect and mechanism of miR-144-3p on radiosensitivity of gastric cancer (GC) cells. Methods Cancer tissue and paracancerous tissue of GC patients admitted to our hospital were collected, their miR-144-3p expression was tested, GC cells were transfected, and survival and biological behavior of those cells under radiation were detected. Results After detection, miR-144-3p expression was down-regulated in GC tissue, while ZEB1 was up-regulated. There was no remarkable difference in the survival fraction of cells in each group before receiving radiation, but that of tumor cells decreased obviously (p < 0.05) after radiation exposure. Survival fraction of cells overexpressing miR-144-3p or silencing ZEB1 decreased more obviously, while the inhibition of miR-144-3p or overexpressing ZEB1 was weaker. Biological behavior of cells under 6 Gy radiation was detected. It was found that miR-144-3p overexpression or silencing ZEB1 dramatically inhibited the proliferation activity of GC cells under 6 Gy radiation, increased the levels of pro-apoptotic Bax and caspase-3 proteins (p < 0.05) and decreased the anti-apoptotic protein Bcl-2 level (p < 0.05), resulting in an increase in the apoptosis rate of cells. miR-144-3p was confirmed to be ZEB1 targeting site by dual luciferase report. Moreover, rescue experiments prove that it can increase the radiosensitivity of GC cells by regulating ZEB1 expression. Conclusion miR-144-3p expression was down-regulated in GC, and it can increase the radiosensitivity of those cells by inhibiting ZEB1 expression (AU)
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Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Tolerancia a Radiación
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Neoplasias Gástricas
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MicroARNs
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Homeobox 1 de Unión a la E-Box con Dedos de Zinc
Límite:
Female
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Humans
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Male
Idioma:
En
Revista:
Clin. transl. oncol. (Print)
Año:
2021
Tipo del documento:
Article