Differential expression of dnaA and dosRgenes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions
Int. microbiol
; 13(1): 9-13, mar. 2010. ilus
Article
en En
| IBECS
| ID: ibc-87667
Biblioteca responsable:
ES1.1
Ubicación: BNCS
ABSTRACT
Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy (AU)
RESUMEN
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Colección:
06-national
/
ES
Base de datos:
IBECS
Asunto principal:
Oxígeno
/
Proteínas Quinasas
/
Proteínas Bacterianas
/
Regulación Bacteriana de la Expresión Génica
/
Proteínas de Unión al ADN
/
Mycobacterium tuberculosis
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Int. microbiol
Año:
2010
Tipo del documento:
Article