Biodegradation characteristics and genomic analysis of a newly isolated indole-degrading strain Pseudomonas aeruginosa Jade-X / Características de biodegradación y análisis genómico de una cepa degradante de indol recientemente aislada Pseudomonas aeruginosa Jade-X
Int. microbiol
; 27(2): 449-457, Abr. 2024. tab, graf
Artículo
en Inglés
| IBECS
| ID: ibc-232292
Biblioteca responsable:
ES1.1
Ubicación: ES15.1 - BNCS
ABSTRACT
Indole is a typical heterocyclic compound derived from tryptophan widespread in nature. Pseudomonas aeruginosa is one of the most common opportunistic pathogens everywhere in the world. Indole and P. aeruginosa will encounter inevitably; however, the indole transformation process by P. aeruginosa remains unclear. Herein, an indole-degrading strain of P. aeruginosa Jade-X was isolated from activated sludge. Strain Jade-X could degrade 1 mmol/L indole within 48 h with the inoculum size of 1% (v/v). It showed high efficiency in indole degradation under the conditions of 3042 °C, pH 5.09.0, and NaCl concentration less than 2.5%. The complete genome of strain Jade-X was sequenced which was 6508614 bp in length with one chromosome. Bioinformatic analyses showed that strain Jade-X did not contain the indole oxygenase gene. Three cytochrome P450 genes were identified and up-regulated in the indole degradation process by RT-qPCR analysis, while cytochrome P450 inhibitors did not affect the indole degradation process. It suggested that indole oxidation was catalyzed by an unraveled enzyme. An ant gene cluster was identified, among which the anthranilate 1,2-dioxygenase and catechol 1,2-dioxygenase genes were upregulated. An indole-anthranilate-catechol pathway was proposed in indole degradation by strain P. aeruginosa Jade-X. This study enriched our understanding of the indole biodegradation process in P. aeruginosa.(AU)
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Texto completo:
Disponible
Colección:
Bases de datos nacionales
/
España
Base de datos:
IBECS
Asunto principal:
Neumonía Viral
/
Infecciones por Coronavirus
/
Asistencia a los Ancianos
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Pseudomonas aeruginosa
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Infecciones por Rickettsia
/
Rifampin
/
Escabiosis
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Esquistosomiasis
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Úlcera Cutánea
/
Aislamiento Social
Límite:
Humanos
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Femenino
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Masculino
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Adulto
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Anciano
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Adolescente
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Niño, preescolar
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Niño
/
Lactante
País/Región como asunto:
Europa
/
America del Sur
/
México
/
Perú
Idioma:
Español
/
Inglés
Revista:
Actas dermo-sifiliogr. (Ed. impr.)
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Allergol. immunopatol
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Clin. transl. oncol. (Print)
/
Enferm. glob
/
Gerokomos (Madr., Ed. impr.)
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Inf. psiquiátr
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Int. microbiol
/
Med. clín (Ed. impr.)
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Med. intensiva (Madr., Ed. impr.)
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Pediatr. aten. prim
Año:
2022
/
2023
/
2024
/
2021
Tipo del documento:
Artículo
/
Congreso y conferencia
Institución/País de afiliación:
Althaia Xarxa Hospitalaria Universitaria de Manresa/España
/
CS Pilarica Circular/España
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CSIC/España
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Centro Sociosanitario Hermanas Hospitalarias Palencia/España
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Dalian Maritime University/China
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Harbin Institute of Technology/China
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Hospital Clínico San Carlos/España
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Hospital Clínico Universitario de Valladolid/España
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Hospital Universitari de Bellvitge/España
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Hospital Universitario Miguel Servet/Spain