Cloning of a B10 DNA puff sequence developmentally amplified and expressed in the salivary gland of Bradysia hygida
Braz. j. med. biol. res
; 25(8): 777-80, 1992. tab, ilus
Article
en En
| LILACS
| ID: lil-113568
Biblioteca responsable:
BR26.1
ABSTRACT
A recombinant clone carrying a 2-kb fragment was isolated from a mini-library of the B10 DNA puff of Bradysia hygida. This fragment was amplified in the salivary gland during the period of DNA puff formation. Amplification started when DNA puff anlage was formed and continued to increase, reaching a maximum of abouth 10-fold 28 h later. Northern blot hybridization experiments showed that this 2-kb fragment was complementary to two RNA species of about 1.3 kb and 1.1 kb, which are developmentally regulated in the salivary gland. Maximum amounts of these messages were present when the B10 puff is fully expanded
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Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Glándulas Salivales
/
Transcripción Genética
/
ADN
/
Amplificación de Genes
/
Clonación Molecular
/
Drosophila
Idioma:
En
Revista:
Braz. j. med. biol. res
Asunto de la revista:
BIOLOGIA
/
MEDICINA
Año:
1992
Tipo del documento:
Article
Pais de publicación:
Brasil