The Pf332 gene codes for a megadalton protein of Plasmodium falciparum asexual blood stages
Mem. Inst. Oswaldo Cruz
; 87(supl.3): 163-8, 1992. ilus
Article
en En
| LILACS
| ID: lil-121095
Biblioteca responsable:
BR15.1
ABSTRACT
We characterized the Plasmodium falciparum antigen 332 (Ag332) which is specifically expressed during the asexual intraerythrocytic cycle of the parasite. The corresponding Pf332 gene has been located in the subtelomeric region of chromosome 11. Furthermore, it is present in all strais so far analyzed and shows marked restriction length fragment polymorphism. Partial sequence and restriction endonuclease digestion of cloned fragments revealed that the Pf332 gene is composed of highly degenerated repeats rich is glutamic acid. Mung been nuclease digestion and Northern blot analysis suggested that Pf332 gene codes for a protein of about 700 kDa. These data were further confirmed by Western blot and immunoprecipitation of parasites extracts with an antiserum raised against a recombinant clone expressing part of the Ag332. Confocal immunofluorescence showed that Ag332 is translocated from the parasite to the surface of infected red blood cells within vesicle-like structures. In addition, Ag332 was detected on the surface of monkey erythrocytes infected with Plasmodium falciparum
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Plasmodium falciparum
/
Polimorfismo Genético
/
Técnica del Anticuerpo Fluorescente
/
Antígenos
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
1992
Tipo del documento:
Article