Medición de IgE total en suero por enzimainmunoanálisis con tres reactivos comerciales / Measurement of total serum IgE by enzyme-immunoanalysis with 3 commercial kits

RESUMO

We measured total serum IgE in 14 patients with allergic diseases and 16 healthy subjects, using 3 commercial ELISA kits. The correlation of results among the 3 kits was analized using Passing and Bablock regression parameters. Results show that measurements of the different kits do not coincide. One kit shows differences using sera from allergic patients. It is concluded that it is not possible to determine exactly the amount of IgE using these kits, specially in subjects with elevated levels