Cellulose acetate as solid phase in ELISA for plague
Mem. Inst. Oswaldo Cruz
; 95(1): 95-6, Jan.-Feb. 2000. graf
Article
en En
| LILACS
| ID: lil-251319
Biblioteca responsable:
BR1.1
ABSTRACT
Antigen from Yersinia pestis was adsorbed on cellulose acetate discs (0.5 cm of diameter) which were obtained from dialysis membrane by using a paper punch. ELISA for human plague diagnosis was carried out employing this matrix and was capable to detect amount of 1.3 µg of antigen, 3,200 times diluted positive serum using human anti-IgG conjugate diluted 14,000. No relevant antigen lixiviation from the cellulose acetate was observed even after washing the discs 15 times. The discs were impregnated by the coloured products from the ELISA development allowing its use in dot-ELISA. Furthermore, cellulose acetate showed a better performance than the conventional PVC plates.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Peste
/
Yersinia pestis
/
Celulosa
/
Antígenos Bacterianos
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2000
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil