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Circulating forms of parathyroid hormone detected with an immunofluorometric assay in patients with primary hyperparathyroidism and in hyperparathyroidism secondary to chronic renal failure
Kunii, I. S; Vieira, J. G. H.
Afiliación
  • Kunii, I. S; Universidade Federal de Säo Paulo. Escola Paulista de Medicina. Divisäo de Endocrinologia. Säo Paulo. BR
  • Vieira, J. G. H; Universidade Federal de Säo Paulo. Escola Paulista de Medicina. Divisäo de Endocrinologia. Säo Paulo. BR
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;34(12): 1547-1550, Dec. 2001. graf
Article en En | LILACS | ID: lil-301408
Biblioteca responsable: BR1.1
ABSTRACT
In patients with uremia, intact parathyroid hormone (PTH) measurement appears to overestimate the biologically active hormone in circulation. The recent description of the accumulation in these patients of a non-intact PTH form measured by the standard immunometric assays, re-opened the question. In this study we submitted serum samples from 7 patients with primary hyperparathyroidism (PHP) and from 10 patients with hyperparathyroidism secondary to chronic renal failure (SHP) to preparative HPLC in order to discriminate the molecular forms measured by our currently used immunofluorometric assay for intact PTH. The elution profile obtained with the HPLC system showed two clearly defined peaks, the first one corresponding to a lower molecular weight form, and the second to the intact PTH (1-84) form. In patients with SHP the area under the curve for the first peak (mean 29.5 percent, range 20.6 to 40.4 percent) was significantly greater than that observed for patients with PHP (mean 15.6 percent, range 5.6 to 21.9 percent). This confirms previous studies showing accumulation of molecular forms of slightly lower molecular weight, presumably PTH (7-84), in patients with SHP and, to a lesser extent, in patients with PHP. The real necessity of assays that discriminate between these two molecular forms is debatable
Asunto(s)
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Hormona Paratiroidea / Hiperparatiroidismo Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2001 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil
Texto completo: 1 Colección: 01-internacional Base de datos: LILACS Asunto principal: Hormona Paratiroidea / Hiperparatiroidismo Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Asunto de la revista: BIOLOGIA / MEDICINA Año: 2001 Tipo del documento: Article / Project document País de afiliación: Brasil Pais de publicación: Brasil