Detection of hepatitis B virus antigens in paraffin-embedded liver specimens from the Amazon Region, Brazil
Mem. Inst. Oswaldo Cruz
; 97(1): 105-107, Jan. 2002. ilus, tab
Article
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| LILACS
| ID: lil-306083
Biblioteca responsable:
BR1.1
RESUMO
Hepatic viscerotomy of paraffin-preserved old specimens, collected in the period from 1934 to 1967, were analyzed by immunohistochemical assays to detect hepatitis B, hepatitis D, dengue and yellow fever virus antigens. The material belongs to the Yellow Fever Collection, Department of Pathology, Instituto Oswaldo Cruz, Rio de Janeiro, Brazil and the cases were diagnosed at that time according to clinical aspects and histopathological findings reporting viral hepatitis, yellow fever, focal necrosis and hepatic atrophy. From the 79 specimens, 69 were collected at the Labrea Region and the other 10 in different other localities in the Amazon Region. The five micra thick histological slices were analyzed for the presence of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) by immunoperoxidase technique. An immunofluorescence assay was applied to the detection of hepatitis D, yellow fever and dengue virus antigens. Nine (11.4 percent) histological samples were HBsAg reactive and 5 (6.3 percent) were HBcAg reactive. The oldest reactive sample was from 1934. Viral antigens related to the other pathologies were not detected in this study. Our results confirm that the methodology described may be used to elucidate the aetiology of hepatitis diseases even after a long time of conservation of the specimens
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Adhesión en Parafina
/
Antígenos del Núcleo de la Hepatitis B
/
Antígenos de Superficie de la Hepatitis B
/
Hígado
Tipo de estudio:
Diagnostic_studies
Límite:
Adolescent
/
Child
/
Child, preschool
/
Female
/
Humans
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Infant
/
Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz
/
Mem. Inst. Oswaldo Cruz (Online)
/
Memórias do Instituto Oswaldo Cruz (Impresso)
/
Proceedings of the Instituto Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil