Teste de DNA para verificaçäo de parentesco em cäes: avaliaçäo do método näo automatizado com o auxílio do primer CMR S / DNA test for parentage verification in dogs: evaluation of the non-automatized method with assistance of the primer CMR S

ABSTRACT

To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and Säo Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use