Characterization of enteroaggregative Escherichia coli strains isolated from children with acute diarrhea, in Mendoza, Argentina
Rev. argent. microbiol
; Rev. argent. microbiol;34(3): 167-170, jul.-sept. 2002.
Article
en En
| LILACS
| ID: lil-331787
Biblioteca responsable:
BR1.1
RESUMO
Enteroaggregative Escherichia coli (EAEC) is an increasingly recognized cause of diarrhea in children in developing and developed countries. EAEC is recognized by a characteristic aggregative pattern of adherence to human epithelial (HEp-2) cells cultured in vitro. This is the gold standard assay. The aggregative phenotype is associated with the presence of a 65 MDa plasmid (pAA) that also encodes several other putative virulence factors, such as the aggregative adherence fimbria I (AAF/I) and the enteroaggregative heat-stable enterotoxin (EAST1). The objective of this work was to evaluate the application of PCR (polymerase chain reaction) to identify EAEC strains in cases of acute diarrhea. A total of 87 E. coli strains, isolated from patients under 2 years of age with acute diarrhea in Mendoza, Argentina, were characterized by the reference method (HEp-2 assay), and by AAF/I- and EAST1-PCR. PCR sensitivity and specificity in comparison with the cell culture assay showed 94.4 sensitivity and 78.26 specificity. EAST1- and AAF/I-PCR could be recommended as a screening test, applicable to epidemiologic studies.
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Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Adhesión Bacteriana
/
Diarrea Infantil
/
Escherichia coli
/
Infecciones por Escherichia coli
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
/
Infant
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Rev Argent Microbiol
/
Rev. argent. microbiol
/
Rev. argent. microbiol. (En línea)
/
Revista argentina de microbiología
Asunto de la revista:
MICROBIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Argentina