Drug susceptibility of Brazilian strains of Mycobacterium bovis using traditional and molecular techniques
Mem. Inst. Oswaldo Cruz
; 99(7): 749-752, Nov. 2004. ilus
Artículo
en Inglés
| LILACS
| ID: lil-391606
Biblioteca responsable:
BR1.1
RESUMO
Transmission of Mycobacterium bovis from cattle to humans has been reported and can cause tuberculosis (Tb) and a problem in certain risk populations. Therefore, knowledge of resistance of M. bovis towards antibiotics used for therapy of human Tb could help avoiding cure delay and treatment cost increase when dealing with drug resistant organisms. We therefore evaluated the susceptibility of M. bovis isolates towards streptomycin, isoniazide, rifampicin, ethambutol, and ethionamide, the first line antibiotics for human Tb. Therefore, 185 clinical samples from cattle with clinical signs of tuberculosis were processed and submitted to culturing and bacterial isolates to identification and drug susceptibility testing using the proportion method. Among 89 mycobacterial strains, 65 were identified as M. bovis and none were resistant to any of the antibiotics used. Confirmation of present results by future studies, enrolling a large number of isolates and designed to properly represent Brazilian regions, may favor the idea of using isoniazide preventive therapy as part of a Tb control strategy in special situations. Also, nucleic acids from bacterial isolates were submitted to rifoligotyping, a recently described reverse hybridization assay for detection of mutations causing resistance towards rifampicin. Concordance between the conventional and the molecular test was 100 percent, demonstrating the use of such methodology for rapid evaluation of drug susceptibility in M. bovis.
Texto completo:
Disponible
Colección:
Bases de datos internacionales
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Enfermedades Desatendidas
/
Tuberculosis
/
Zoonosis
Base de datos:
LILACS
Asunto principal:
Pruebas de Sensibilidad Microbiana
/
Mycobacterium bovis
/
Antituberculosos
Tipo de estudio:
Estudio observacional
/
Factores de riesgo
Límite:
Animales
/
Humanos
País/Región como asunto:
America del Sur
/
Brasil
Idioma:
Inglés
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
Medicina Tropical
/
Parasitología
Año:
2004
Tipo del documento:
Artículo
/
Documento de proyecto
País de afiliación:
Brasil
Institución/País de afiliación:
Fiocruz/BR
/
Instituto Oswaldo Cruz-Fiocruz/BR
/
Ministério da Agricultura, Pecuária e Abastecimento/BR
/
Universidade Federal de Minas Gerais/BR
/
Universidade Federal do Rio de Janeiro/BR