A comparative analysis of highly conserved sex-determining genes between Apis mellifera and Drosophila melanogaster
Genet. mol. res. (Online)
; 5(1): 154-168, Mar. 31, 2006. ilus, tab
Article
en En
| LILACS
| ID: lil-449136
Biblioteca responsable:
BR1.1
ABSTRACT
A comparison of the most conserved sex-determining genes between the fruit fly, Drosophila melanogaster, and the honey bee, Apis mellifera, was performed with bioinformatics tools developed for computational molecular biology. An initial set of protein sequences already described in the fruit fly as participants of the sex-determining cascade was retrieved from the Gene Ontology database (http//www.geneontology.org/) and aligned against a database of protein sequences predicted from the honey bee genome. The doublesex (dsx) gene is considered one of the most conserved sex-determining genes among metazoans, and a male-specific partial cDNA of putative A. mellifera dsx gene (Amdsx) was identified experimentally. The theoretical predictions were developed in the context of sequence similarity. Experimental evidence indicates that dsx is present in embryos and larvae, and that it encodes a transcription factor widely conserved in metazoans, containing a DM DNA-binding domain implicated in the regulation of the expression of genes involved in sexual phenotype formation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Abejas
/
Genes de Insecto
/
Secuencia Conservada
/
Biología Computacional
/
Procesos de Determinación del Sexo
/
Drosophila melanogaster
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Genet. mol. res. (Online)
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2006
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Brasil