Duplex-PCR assay for the detection of adenovirus and respiratory syncytial virus in nasopharyngeal samples
Mem. Inst. Oswaldo Cruz
; 104(1): 118-120, Feb. 2009. ilus
Article
en En
| LILACS
| ID: lil-507216
Biblioteca responsable:
BR1.1
ABSTRACT
Human adenovirus (HAdV) and human respiratory syncytial virus (HRSV) are important etiologic agents of acute respiratory infections. In this study, a duplex polymerase chain reaction (PCR) assay was developed for the simultaneous detection of HAdV and HRSV in clinical samples. Sixty previously screened nasopharyngeal aspirates were used 20 HAdV-positive, 20 HRSV-positive and 20 double-negative controls. Eight samples were positive for both viruses. The duplex PCR assay proved to be as sensitive and specific as single-target assays and also detected the mixed infections with certainty. The identification of both viruses in a single reaction offers a reduction in both cost and laboratory diagnostic time.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
LILACS
Asunto principal:
Infecciones del Sistema Respiratorio
/
Adenoviridae
/
Nasofaringe
/
Virus Sincitial Respiratorio Humano
/
Infecciones por Virus Sincitial Respiratorio
/
Infecciones por Adenoviridae
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Límite:
Child
/
Humans
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Asunto de la revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Año:
2009
Tipo del documento:
Article
/
Project document
País de afiliación:
Brasil